氯化铟对大鼠骨髓嗜多染红细胞微核的影响  

Genotoxic damage induced by indium chloride on rat

作　　者：石英杰 康惠文 王可心 朱睿 常志敏 刘楠 关维俊 李斌[2] 

机构地区：[1]华北理工大学公共卫生学院,唐山063000 [2]中国疾病预防控制中心职业卫生与中毒控制所

出　　处：《卫生研究》2016年第6期973-976,997,共5页Journal of Hygiene Research

基　　金：国家公益性卫生行业科研专项(No.201402021);华北理工大学大学生创新性实验计划项目(No.X2015163)

摘　　要：目的研究氯化铟气管注入染毒对大鼠造成的遗传损伤和氧化损伤。方法选取32只雄性健康成年SPF级Wistar大鼠,随机分为4组[生理盐水对照组,低、中、高剂量(0.065、0.65和1.3 mg/kg)氯化铟染毒组],采用非暴露式气管内注入法染毒,HE染色法光镜下观察肺组织病理形态学变化,测定肺组织匀浆丙二醛(MDA)和超氧化物歧化酶(SOD)含量,对骨髓涂片进行嗜多染红细胞微核计数,并采用ICP-MS法检测大鼠全血和肺组织中铟的含量。结果 3个剂量组大鼠血铟、肺铟含量和骨髓嗜多染红细胞微核率均明显高于对照组(P<0.01);中、高剂量组大鼠肺组织中MDA含量亦较对照组显著增加(P<0.05),而SOD水平均显著低于对照组(P<0.05);肺组织病理观察显示氯化铟染毒后大鼠气管周围充血明显,肺泡间隔、细支气管壁增厚,肺泡腔内充满细颗粒状蛋白样物质。结论氯化铟气管注入法染毒会引起大鼠血铟、肺铟蓄积,骨髓嗜多染红细胞微核率增加,发生氧化损伤和组织病理学改变。Objective To investigate the genotoxic effect of indium chloride using the in vivo bone marrow micronucleus test,and to provide scientific basis for the evaluation of the toxicity of indium compounds.Methods Thirty two healthy male adult SPF Wistar rats were selected and randomly divided into four groups（ control group,0.065,0.65,1.3 mg/kg group）.Rats were treated with indium chloride using non exposed endotracheal injection method.Hematoxylin and eosin（ H＆E） staining was used to detect histological changes.Oxidative stress status relative to lung damage in rats was evaluated by detecting superoxide dismutase（ SOD） and maleic dialdehyde（ MDA） activities.The frequency of micronuclei was determined at the end of each culture period.Inductively coupled plasma mass spectrometry（ ICP-MS） method was used to detect the content of indium in whole blood and lung tissue of rats.Results The content of indium in whole blood and lung tissue of rats and the micronucleus rate in rats exposed to indium chloridewere significantly higher than those in the control group（ P 〈 0.01）.The MDA content in the lung tissue of rats in middle and high dose group was significantly increased（ P 〈0.05）,while SOD level was significantly lower than that in control group（ P 〈 0.05）;Pathological changes of lung tissue showed that after exposure to indium chloride,inflammatory cell exudation was present in the alveolar cavity and surrounding areas of the trachea were apparent.Alveolar lumen was filled with intraalveolar proteinaceous material.Conclusion Indium chloride exposure will cause the accumulation of indium in blood and lung,bone marrow micronucleus rate increased,occurrence of oxidative damage and pathological changes.

关 键 词：铟 遗传损伤 微核 

分 类 号：R135.2[医药卫生—劳动卫生] Q593.6[医药卫生—公共卫生与预防医学]