芹菜素对人胶质瘤SHG-44细胞增殖抑制和诱导凋亡的作用  

Apigenin inhibits human glioma SHG-44 cells growth and induces apoptosis

作　　者：徐旋旋 石磊[2] 张曙光[2] 潘天鸿[2] 

机构地区：[1]江苏大学医学院,212000 [2]江苏大学附属昆山市第一人民医院神经外科

出　　处：《江苏医药》2016年第22期2421-2424,F0002,共5页Jiangsu Medical Journal

基　　金：国家自然科学基金(1603240005);江苏大学科研项目(JLY20140045)

摘　　要：目的研究芹菜素对体外人胶质瘤SHG-44细胞增殖抑制和诱导凋亡的作用。方法MTT法检测细胞增殖抑制率。Hoechst 33258和Tunel染色法观察细胞凋亡的形态学变化。AnnexinVFITC/PI双染流式细胞术检测不同浓度芹菜素处理后细胞凋亡率。流式细胞术检测细胞周期。Western blot检测凋亡相关蛋白Bax和Bcl-2的表达变化。结果芹菜素能抑制SHG-44细胞的增殖,并呈时间和剂量依赖性(P<0.05)。芹菜素处理组细胞出现典型细胞凋亡特征。芹菜素可使SHG-44细胞阻滞在G_2/M期。不同浓度的芹菜素作用24h,细胞凋亡率逐渐增加,呈浓度依赖性。芹菜素处理组细胞的Bax蛋白表达量随药物浓度的增加而增加,Bcl-2蛋白表达量随药物浓度的增加而降低。结论芹菜素能抑制胶质瘤细胞增殖,并可通过线粒体信号途径诱导胶质瘤细胞凋亡。Objective To investigate the effects of apigenin on human glioma SHG-44 cells growth and inducing apoptosis in vitro. Methods The cell proliferation was detected by MTT assay, cell cycle was measured by flow eytometry, and the apoptosis of SHG-44 cells was observed under fluorescence microscope by Hoechst 33258 staining and Tunel staining, which was quantified by flow cytomentry using AnnexinV-FITC/PI double stain. The expressions of apoptosis-related protein Bax and Bcl-2 were analyzed by Western blot. Results Apigenin inhibited the proliferation of the cells in concentration- and time-dependent manners （P〈0. 05 ）. The SHG-44 cells treated with apigenin showed significant morphological changes of apoptosis. At 24 hours after apigegin treatment, the SHG-44 cells were arrested in G2/M phase with increased cell apoptosis concentration-dependently. The up-regulation of Pax and down-regulation of Bcl-2 were induced by apigenin. Conclusion Apigenin can inhibit cell proliferation and induce apoptosis of human glioma cell line SHG-44 via activating mitochondrial signal pathway.

关 键 词：芹菜素 胶质瘤 细胞增殖 细胞凋亡 

分 类 号：R651[医药卫生—外科学]