温肾活血方改善胚胎着床障碍小鼠子宫内膜容受性的研究  

Experimental research of Wenshen Huoxue decoction in the improvement of endometrial receptivity in mouse model of embryo implantation dysfunction

作　　者：张琦[1] 张勤华[2] 贾亚楠[1] 

机构地区：[1]上海中医药大学,上海201203 [2]上海中医药大学附属曙光医院生殖中心,上海201203

出　　处：《生殖与避孕》2016年第11期875-881,共7页Reproduction and Contraception

基　　金：国家自然青年科学基金项目;项目号:81202715

摘　　要：目的：探讨温肾活血方对胚胎着床障碍模型小鼠妊娠结局及子宫内膜容受性的影响。方法：由米非司酮建立胚胎着床障碍模型小鼠200只，随机分为正常（N）组、模型（M）组、中药低剂量（L）组、中药高剂量（H）组和孕酮（P）组各40只。妊娠第1～4日早晨，H组、L组小鼠灌胃给予0．3mL水煎剂，N组、M组小鼠每日以等体积生理盐水灌胃，P组小鼠以配制的黄体酮－橄榄油剂按照2mg／只进行颈后皮下注射，妊娠第4日上午9：00造模，N组4、鼠颈后皮下注射0．1mL橄榄油溶剂，其他各组小鼠颈后皮下注射米非司酮－橄榄油剂0．1mL（1．93mg／kg米非司酮）。分别于妊娠第4日、第5日、第8日上午9：00脱颈椎处死每组各10只小鼠，检测妊娠率、着床位点数、子宫内膜组织形态、雌激素（E2）及其受体（ER）、孕激素（P）及其受体（PR）、胞饮突。结果：N组着床位点数多于其它各组，H组与N组比较无统计学差异（P〉0.05）。子宫内膜发育水平N组最优，H组接近N组，M组滞后。妊娠第4日，N组的E2、P和ER、PR的表达水平与M组比较无统计学差异（P〉0．05），低于P组与H组（P〈0．01）：妊娠第5日，N组的E2、P和ER、PR的表达水平显著高于M组（P〈0．05），N组的ER水平与P、H、L三组比较无统计学差异（P〉0.05），N组的PR高于P组和L组（P〈0．05），与H组比较均无统计学差异（P〉0．05）。P组P水平高于其它组（P〈0．01），E2水平与ER结果相吻合。M组胞饮突发育滞后，H组胞饮突发育程度接近N组，P组偶见退化期胞饮突。结论：温肾活血方能够改善子宫内膜容受性，且其效果优于黄体酮。Objective： To explore the effects of Wenshen Huoxue decoction on pregnancy outcome and endometrial receptivity for mouse model of embryo implantation dysfunction. Methods： A total of 200 mice of embryo implantation dysfunction model built by mifepristone were randomized into normal （hi） group, model （M） group, low dose （L） group of Wenshen Huoxue decoction, high dose （H） group of Wenshen Huoxue decoction and progesterone （P） group, 40 mice in each group. From day 1 to 4, groups H and L were given the dose of high or low of 0.3 mL Wenshen Huoxue decoction everyday, groups N and M were daily filled with equal volume of normal saline, and group P was given 0.2 mL of the prepared P-olive oil via neck subcutaneous injection. To bulid mouse model, group N was injected with 0.1 mL olive oil in neck subcutaneous in the morning of 9 o＇clock olD4, other mice in each group were injected with 0.1 rnL mifepristone-olive oil （1.93 mg/kg mifepristone）. Ten mice of each group were sacrificed at 9 o＇clock on day 4, 5 and 8 of pregnancy respectively. Pregnancy rate, the number of implantation sites, morphology of endometrial tissue, estrogen （E2）, estrogen receptor （ER）, P, progesterone receptor （PR） and the pinopode were detected. Results： For implantation sites： group H was not significantly different with group N （P〉0.05）, and group N was better than other groups. For morphology of endometrial tissue： the endometrial development in group M laged behind other groups, especially for group N, but group H was close to group N. For the level of E2, P, ER and PR： on D4 of pregnancy, there was no significant difference between groups N and M （P〉0.05）, and they were less in groups N and M than in groups P and H （P〈0.01）; on D5 of pregnancy, they were higher in group N than in group M （P〈0.05）. Compared with groups P, H or L, the ER level in group N was not significantly different from ER （P〉0.05）. PR level in group N was higher than that in groups P and L

关 键 词：温肾活血 胚胎着床障碍 子宫内膜容受性 胞饮突 

分 类 号：R285.5[医药卫生—中药学]