寡霉素处理下细胞外ATP和细胞内ATP的关系及其对细胞死亡调节作用的研究  

Relationship between Extracellular ATP and Intracellular ATP and Its Role in Regulating the Cell Death under Oligomycin Treatment

作　　者：王庆文[1] 白晶月 石岱龙 贾凌云[1] 冯汉青[1] 

机构地区：[1]西北师范大学生命科学学院,兰州730070

出　　处：《植物研究》2016年第6期925-930,共6页Bulletin of Botanical Research

基　　金：国家自然科学基金(31260059;31560070);甘肃高校基本科研业务费~~

摘　　要：以烟草悬浮细胞BY^(-2)为材料,利用FoF1-ATP合成酶(FoF1-ATPase)抑制剂寡霉素研究了细胞内ATP(i ATP)对胞外ATP(e ATP)水平的影响,以及施加外源ATP对i ATP水平和细胞死亡的调节作用。结果表明,随着寡霉素浓度的增加(5、10、25、50μmol·L^(-1)),烟草悬浮细胞的i ATP含量逐渐下降,e ATP含量也随之减少,且细胞死亡水平在较高寡霉素处理浓度下有明显上升。同时,随着寡霉素处理时间的增加(0.5、1、3、5 h),烟草悬浮细胞的i ATP含量和e ATP含量也呈现出不同程度的下降,而细胞死亡水平则有所增加。施加外源ATP能够缓解寡霉素引起的细胞i ATP水平的下降和细胞死亡水平的上升。上述结果表明,e ATP水平受到了i ATP水平的影响,而e ATP也在线粒体ATP合成受到抑制时调控i ATP和细胞死亡的发生。In BY-2） tobacco suspension cells,the effect of intracellular ATP（ i ATP） production on extracellular ATP（ e ATP） levels was studied by using oligomycin（ Inhibitors of F_oF_1-ATPase）. The results showed that with the increase of the concentrations of oligomycin（ from 5- 50 μmol·L^（-1））,the level of i ATP and e ATP were decreased,and the level of cell death were increased under higher concentration of oligomycin. With the increase of the times of oligomycin treatment（ 0. 5,1,3 and 5 h）,the levels of both i ATP and e ATP were decreased in different extents,while the level of cell death were increased. Treatment with exogenous ATP alleviated the decrease in i ATP and the increase in cell death level that were caused by oligomycin,The level of e ATP can be affected by i ATP,and e ATP can regulate the i ATP production and cell death under the condition of mitochondrial oxidative phosphorylation being inhibited.

关 键 词：胞内ATP 胞外ATP 寡霉素 细胞死亡 

分 类 号：Q945.79[生物学—植物学]