哮喘大鼠嗜铬蛋白A表达的检测及意义  

Detection and significance of chromogranin A in asthmatic rat

作　　者：童夏生[1] 范广民[2] 蒋珍凤 丁珺云 王恩智[5] 

机构地区：[1]台州市中西医结合医院儿科,浙江台州317500 [2]台州市中心医院病理科,浙江台州317500 [3]温岭市卫生进修学校内科,浙江台州317500 [4]温岭市第四人民医院内科,浙江台州317500 [5]台州市中西医结合医院检验科,浙江台州317500

出　　处：《中国卫生检验杂志》2016年第21期3072-3074,共3页Chinese Journal of Health Laboratory Technology

基　　金：温岭市科技局基金资助项目(2012-2-109)

摘　　要：目的探讨嗜铬蛋白A在哮喘发病机制中的作用。方法通过哮喘大鼠模型,分别采用免疫组化法和实时荧光定量PCR法检测肺组织和血中性粒细胞(PMN)嗜铬蛋白A或mRNA的表达。结果哮喘组肺组织嗜铬蛋白A及mRNA的表达水平显著高于对照组,差异有统计学意义(P<0.01);布地奈德组嗜铬蛋白A及mRNA的表达水平显著低于哮喘组,差异有统计学意义(P<0.01)。哮喘组血PMN嗜铬蛋白A mRNA的表达量显著高于对照组,差异有统计学意义(P<0.01),布地奈德组嗜铬蛋白A mRNA的表达量与哮喘组差异无统计学意义(P>0.05)。结论哮喘大鼠嗜铬蛋白A的表达升高,嗜铬蛋白A可能参与了哮喘的炎症机制,其表达能被布地奈德所抑制。Objective To investigate the potential roles of chromogranin A in asthma pathogenesis. Methods The expressions of chromogranin A or mRNA were detected by immunohistochemisty or real- time quantitative polymerase chain reaction methods at lung tissue and blood neutrophil of asthmatic rat model. Results Levels of chromogranin A and mRNA at lung tissue in asthma group were significantly higher than those in control group,with the differences statistically significant（ P〈0. 01）; that in the budesonide group was significantly lower than that in the asthma group（ P〈0. 01）. And levels of chromogranin A mRNA at blood neutrophil in asthma group were significantly higher than that in control group,with the difference statistically significant（ P〈0. 01）,but it had no statistical significance on the difference of chromogranin A mRNA between in budesonide group and asthma group（ P〉0. 05）. Conclusion Levels of chromogranin A increased in asthmatic rats,the results showed chromogranin A might participate in asthma inflammation,but this function can be inhibited by budesonide.

关 键 词：哮喘 嗜铬蛋白A 布地奈德 发病机制 实时荧光定量PCR 免疫组化 

分 类 号：R562.25[医药卫生—呼吸系统]