microRNA-499靶控的PI3K/Akt通路在大鼠缺血-再灌注心肌损伤中的作用  

Effect of microRNA-499 targeted at PI3K/Akt signaling pathway on myocardial ischemia-reperfusion in rats

作　　者：崔瑞新[1] 吕风华[1] 岳莹[1] 刘亚坤[1] 高建芝[1] 

机构地区：[1]新乡医学院第一附属医院心内一科,新乡453100

出　　处：《郑州大学学报（医学版）》2016年第6期740-743,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省高等学校青年骨干教师资助计划2009GGJS-085

摘　　要：目的:探讨microRNA-499在大鼠心肌缺血-再灌注损伤(MIRI)中的作用及其与PI3K/Akt通路的关系。方法:将30只SD大鼠随机分为假手术组、模型组和干预组,每组10只。假手术组仅在冠状动脉的左前降支下穿线但并不结扎,模型组结扎冠状动脉左前降支以建立大鼠MIRI模型(缺血30 min,再灌注120 min),干预组于建立MIRI模型前48 h注射microRNA-499激动剂。采用荧光定量RT-PCR法检测大鼠心肌组织中miRNA-499及PI3K mRNA表达量,Western blot法检测Akt磷酸化活性,采用TTC染色法检测心肌梗死面积。结果:与假手术组相比,模型组miRNA-499及PI3K mRNA表达量减少,Akt磷酸化活性明显降低,心肌梗死面积明显增加(P<0.05)。与模型组比较,干预组miRNA-499及PI3K mRNA表达量、Akt磷酸化活性明显升高,心肌梗死面积明显减少(P<0.05)。心肌组织中PI3K mRNA与microRNA-499的表达量呈正相关(P<0.05)。结论:microRNA-499可能通过激活PI3K/Akt信号通路,减轻大鼠MIRI。Aim： To investigate the effect of miRNA-499 on myocardial ischemia-reperfusion injury（ MIRI） of rats and its relation with PI3 K / Akt signaling pathway. Methods： Thirty SD rats were randomly assigned into sham-operation group,model group,and precondition group with 10 rats in each group. In the sham-operation group,the thread was only threaded under left anterior descending coronary artery（ LAD） but not ligated; in model group,MIRI models were built by ligating LAD with 30 min ischemia and 120 min reperfusion; in the precondition group,rats were injected miRNA-499 agomir 48 h before MIRI operation.Real Time RT-PCR was adopted to detect the expressions of miRNA-499 and PI3 K mRNA,Western blot was adopted to detect Akt phosphorylation level,and the myocardial infarction size were measured using TTC staining. Results： Compared with shamoperation group,the expressions of miRNA-499 and PI3 K mRNA,and the Akt phosphorylation level in model group were significantly lower,and the infarction size were larger（ P〈0. 05）. Compared with those in MIRI group,the expressions of miRNA-499 and PI3K mRNA,and the Akt phosphorylation level in precondition group were obviously higher,while the infarction size was smaller（ P〈0. 05）. The expression of microRNA-499 was positively related to that of PI3 K mRNA（ P〈0. 05） 。Conclusion： microRNA-499 could reduce MIRI via activating the PI3 K / Akt signaling pathway.

关 键 词：心肌 缺血-再灌注损伤 microRNA-499 PI3K Akt 大鼠 

分 类 号：R542.2[医药卫生—心血管疾病]