褪黑素对急性脊髓损伤大鼠脊髓组织中MPO及CINC-1表达的影响  

Effects of melatonin on expression of MPO and CINC-1 in rats with acute spinal cord injury

作　　者：苗金红[1] 朱海洋[2] 钟斌[2] 崔浩[2] 汪鑫[2] 理阳 徐玉生[2] 

机构地区：[1]郑州大学第一附属医院,郑州450052 [2]郑州大学第一附属医院骨科,郑州450052

出　　处：《郑州大学学报（医学版）》2016年第6期757-761,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省国际科技合作项目134300510005

摘　　要：目的:探讨褪黑素(MT)对大鼠急性脊髓损伤后髓过氧化物酶(MPO)及中性粒细胞趋化因子(CINC)-1表达的影响。方法:108只SD大鼠分为假手术组、脊髓损伤组、褪黑素治疗组。手术组大鼠仅切除T11椎板,脊髓损伤组、褪黑素治疗组建立T11脊髓损伤模型。脊髓损伤组、褪黑素治疗组在脊髓损伤后10 min内分别腹腔注射含体积分数5%乙醇的生理盐水和100 mg/kg的褪黑素制剂。于脊髓损伤后1 h、6 h、12 h、1 d、3 d、5 d分别取脊髓组织,化学法测定MPO活力变化,HE染色法观察脊髓组织结构,RT-PCR法观察CINC-1 mRNA表达情况。结果:脊髓内CINC-1水平和MPO活性有相似的变化规律,且MPO在CINC-1表达达峰之后12 h达高峰;除1 h外各时间点各组相比较,B组最高、C组次之、A组最低,其中B组与A组、C组差异具有统计学意义(P<0.05)。结论:褪黑素可通过抑制CINC-1的表达减少中性粒细胞的浸润,从而减轻炎症反应,起到一定的神经保护作用。Aim： To investigate the effects of melatonin（ MT） on expression of myeloperoxidase（ MPO） and cytokineinduced neutrophil chemoattractant（ CINC）-1 in rats after acute spinal cord injury. Methods： Totally 108 SD rats were randomly allocated into 3 groups： sham operation group（ group A,n = 36）,spinal cord injury group（ group B,n = 36）,and MT treatment group（ group C,n = 36）. Group B and C were established SCI animal models at T11 level. Lamina of vertebra was cut without spinal cord injury in the sham operation group. The rats of group C were injected 5%（ V / V） alcohol into abdominal cavity after spinal cord injury while the rats of group B were injected MT（ 100 mg / kg）. Fetching the spinal tissue at 1 h,6 h,12 h,1 d,3 d and 5 d after spinal cord injury. Chemical method was used to detect the expression of MPO. HE staining was performed to observe the pathological changes of the spinal cord in the 3 groups respectively. RTPCR was used to detect the expression of CINC-1 mRNA in spinal cord tissue. Results： There were similar changing patterns of contents of CINC-1 and MPO,and the peak time of MPO was 12 h later than CINC-1. Compared the three groups except 1 h,group B had the highest level and group A had the lowest level regarding to the contents of CINC-1 and MPO,and the differences among them were statistical（ P〈0. 05）. Conclusion： MT could inhibit the expression of CINC-1 and reduce neutrophil infiltration,thus relieve inflammation reaction and protect spinal cord from damage.

关 键 词：褪黑素 脊髓损伤 中性粒细胞趋化因子 髓过氧化物酶 大鼠 

分 类 号：R681.54[医药卫生—骨科学]