苹果‘长富2号’开花调控转录因子基因MdSPL6的克隆及表达分析  

Cloning and Expression of the Flowering Regulation Transcription Factor Gene MdSPL6 in Malus × domestica

作　　者：樊胜[1] 张岚清[1] 刘柯[1] 雷超[1] 陈欣[1] 姚殿城 张东[1] 韩明玉[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《园艺学报》2016年第11期2089-2098,共10页Acta Horticulturae Sinica

基　　金：国家现代农业产业技术体系建设专项资金项目(CARS-28);国家自然科学基金项目(31672101);陕西省科技统筹项目(2015NY114;2016KTZDNY01-10);国家苹果改良中心杨凌分中心项目;陕西省果业发展协同中心项目;大学生创新训练计划项目

摘　　要：以‘长富2号’苹果短枝顶芽为材料,采用RT-PCR方法克隆得到1个候选开花调控转录因子基因SQUMOSA PROMOTER BINDING PROTEIN LIKE(SPL),含有570 bp开放阅读框,编码189个氨基酸。序列比对发现,该基因含有一个保守的SBP结构域和核定位信号,属于SBP转录因子基因家族。系统进化分析表明,MdSPL6蛋白的核苷酸序列与拟南芥AtSPL3、AtSPL4和AtSPL5具有较高的同源性。实时定量PCR分析结果表明,在不同的组织中MdSPL6的表达量存在差异,在叶片和顶芽中的表达量相对较高。易成花的‘烟富6号’苹果短枝顶芽内MdSPL6的表达高于难成花的‘长富2号’。外源GA处理诱导MdSPL6的表达在花后40和60 d下调;6-BA处理使MdSPL6的表达呈现先上升后下降趋势;蔗糖处理促使MdSPL6表达上调。推测MdSPL6对激素和糖信号介导的成花诱导有重要作用,可作为调控苹果花芽分化的目标基因。One important blossom transcription factor SQUMOSA PROMOTER BINDING PROTEIN LIKE （SPL） was separated and cloned by RT-PCR from the buds on the top of spurs in ＇Fuji＇ apple tree. The open reading frame of MdSPL6 was 570 bp, encoding a protein of 189 amino acids. Sequence alignment analysis showed that MdSPL6 shared a highly conserved SBP domain and bipartite nuclear localization signal （NLS）, and it belonged to the SBP family. Additional, there were high homology of amino acid sequences between MdSPL6 and AtSPL3, AtSPL4, AtSPL5. The results showed that, the transcription level of MdSPL6 was different among the stem, leaf, flower, fruit and bud, and it showed higher level in buds and leaves. MdSPL6 was also higher in the buds of ＇Yanfu 6＇ than in ＇Changfu 2＇.Meanwhile, exogenous GA repressed the expression of MdSPL6 at 40 and 60 DAFB, while showed upgrade and then descending after exogenous 6-BA treatment. Additional, the expression of MdSPL6 increased after exogenous sugar treatment. MdSPL6 plays important roles in hormone and sugar mediated flowering, which may be used as a target gene in regulation of apple flower induction.

关 键 词：苹果 成花诱导 MdSPL6 克隆 基因表达 

分 类 号：S661.1[农业科学—果树学]