氰氨化钙土壤消毒对黄瓜根腐病及土壤病原菌的控制效果  

Studies of Soil Improvement Effect of Calcium Cyanamide and Its Control Efficiency on Soil-borne Diseases of Vegetable Crops

作　　者：贲海燕[1] 石延霞[2] 谢学文[2] 柴阿丽[2] 李宝聚[2] 

机构地区：[1]黑龙江省农业科学院园艺分院,哈尔滨150069 [2]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《园艺学报》2016年第11期2173-2181,共9页Acta Horticulturae Sinica

基　　金：国家科技支撑计划项目(2012BAD19B06);中国农业科学院创新工程项目(CAAS-ASTIP-IVFCAAS);农业部园艺作物生物学与遗传育种重点实验室项目;公益性行业(农业)科研专项项目(201503109)

摘　　要：应用氰氨化钙土壤消毒技术防治黄瓜根腐病,通过病害防治效果、植株生长情况等指标,结合土壤中茄病镰刀菌实时荧光定量PCR检测手段明确氰氨化钙不同剂量处理后病原菌菌量的动态变化,以此评估氰氨化钙土壤消毒防治黄瓜根腐病的施用效果。应用建立优化的荧光定量PCR检测体系监测土壤中茄病镰刀菌带菌量,最低检测量为1×101个拷贝·g^(-1)。研究结果表明,氰氨化钙最佳施药量为120 g·m^(-2),其茄病镰刀菌消毒3 d菌量大幅下降,9 d时降为0,种植黄瓜苗后菌量恢复缓慢,对黄瓜根腐病防治效果为66.56%~74.41%,植株长势良好。This paper reported the effects of calcium cyanamide （CaCN2） on control of cucumber root rot caused by Fusarium solani. Through the examination of disease control effectiveness, plant growth and other indices, we evaluated the antimicrobial effects of CaCN2 on E solani in soil at different doses and different treatment periods by real-time PCR. The minimal detectable concentration of 1 × 10^1 dRn · g-1 soil was established to monitor the population of E solani. The results showed that CaCN2 reached its best inhibitory effects on E solani at the dose of 120 g · m-2. In disinfection period, the population of pathogen greatly reduced within 3 days, and little pathogen could be detectable at the 9th day. In plant growing period, the population of this pathogen recovered slowly, and the control efficiency could reach 66.56% - 74.41%.

关 键 词：黄瓜 氰氨化钙 茄病镰刀菌 防治效果 Real-time PCR 动态监测 

分 类 号：S642.2[农业科学—蔬菜学]