NaCl、蔗糖和KH_2PO_4对红叶桃离体叶片花色苷合成基因表达的影响  

NaCl, Sucrose and KH_2PO_4 Effects the Expression of Anthocyanins Synthesis Gene on Red-leaf Peach in vitro

作　　者：文杨[1] 韩键[1] 姜卫兵[1] 王小青[1] 房经贵[1] 张斌斌[2] 马瑞娟[2] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]江苏省农业科学院园艺研究所,南京210014

出　　处：《园艺学报》2016年第11期2214-2222,共9页Acta Horticulturae Sinica

基　　金：江苏省优势学科建设项目(2011PAPD);国家现代农业产业技术体系建设专项资金项目(CARS-31)

摘　　要：为探明外源化学物质对红叶桃叶片着色的影响及其机制,以‘筑波5号’离体叶片为试材,研究不同浓度NaCl、蔗糖及KH_2PO_4处理对叶片中花色苷生物合成途经相关基因表达水平的影响。结果显示:低浓度NaCl(100和200 mmol′L^(-1))短时间(4 h)处理能提高花色苷合成结构基因CHI、UFGT和转录因子基因MYB10、bHLH33的表达水平;0.3%蔗糖处理8 h对UFGT、MYB10和bHLH33的表达促进作用显著;0.2%KH_2PO_4处理8 h对CHI、UFGT、MYB10、bHLH33和WD40的表达也具有显著的促进作用,而且CHI的表达在一定范围内随蔗糖及KH_2PO_4浓度增大和处理时间延长,效果更明显。除了0.2%KH_2PO_4处理8 h提高了WD40的转录外,NaCl、蔗糖及KH_2PO_4对MYB15和WD40的表达均具有显著抑制作用。The leaves of ＇Zhubo-5＇ peach in vitro were as the test materials, using the fluorescent quantitative PCR technology to analysis related gene expression level of anthocyanins biosynthesis via different concentrations of NaCl, sucrose and KH2PO4 treatment. The result showed, NaCl treatment can improve the expression level ofCHI, UFGT, MYBIO, bHLH33 genes, and suppress MYB15 and WD40 genes expression. For CHI gene, sucrose and KHEPO4 treatment can significantly improve its expression, and within a certain range, the expression levels were positively correlated with concentration and time. The expression level of UFGT in three concentrations of sucrose began to increase after 8 h, the concentration of 0.3% was the best. MYBIO gene transcription level increased by sugar processing, consistent with the CHI. Concentration of 0.8% sucrose treatment decreased bHLH33 expression, concentration of 0.3% and 0.5%, after 8 h showed positive regulation function, and in 0.3% 8 h was the best effect. KH2PO4 treatment improved UFGT, MYB10, bHLH33 and WD40 expression at 0.2%, and the peak-expression was after 8 h treatment. In this experiment, sucrose was inhibitory effected on WD40 gene. The result of this study was to further clarify the mechanism of anthocyanins synthesis and provide certain theoretical basis to improve ornamental period.

关 键 词：红叶桃 荧光定量PCR 花色苷生物合成 基因表达 

分 类 号：S662.1[农业科学—果树学]