端粒长度检测方法及其应用  

Methods of Measuring Telomere Length and Its Application

作　　者：张彬[1] 王长利[1] 

机构地区：[1]天津医科大学肿瘤医院肺部肿瘤科国家肿瘤临床医学研究中心天津市肿瘤防治重点实验室,天津300060

出　　处：《生物技术通报》2016年第11期93-98,共6页Biotechnology Bulletin

基　　金：天津市抗癌重大专项(12ZCDZSY15400)

摘　　要：人类的衰老与肿瘤的发生是影响人寿命的重要因素,几十年来的研究表明端粒长度的变化在这两个进程中扮演了极其重要的角色,因此熟知分析端粒长度的方法就显得非常重要。目前,有多种端粒长度检测的方法,包括端粒末端限制性片段分析(terminal restriction fragment,TRF),定量PCR(q PCR),荧光原位杂交(fluorescence in situ hybridization,FISH)和单链端粒长度分析(single telomere length analysis,STELA)等。但每种方法都有适用的范围,在研究和应用过程中需要熟知各种方法的原理、优势和局限,从而选择合适的检测方法,才能达到我们的研究目的,解决具体问题。因此将对目前常用的端粒长度检测方法进行综述。Aging and tumor are the two main factors influencing human longevity. Many studies have proved that telomere play importantroles in these progresses. Thus, it is critical to familiarize with assay methods of telomere length. The most commonly used methods includetelomere restriction fragment（TRF）length analysis, quantitative PCR（qPCR）, fluorescent in situ hybridization（FISH）and singletelomere length analysis（STELA）. However, each method has the scope of application, in the process of research and application, we have tounderstand the principle of each method, its advantages and disadvantages, then we may choose the appropriate detection method to achieve theaim of our study and solve specific issues. The commonly used detection methods of telomere length are summarized here.

关 键 词：端粒长度 端粒末端限制性片段分析 定量PCR 荧光原位杂交 单链端粒长度分析 

分 类 号：Q78[生物学—分子生物学]