不同方法检测奶牛结核病的结果分析  

Results Analysis of Cow Tuberculosis Detection by Different Methods

作　　者：张洪军 王会敏 李秀慧 张秀丽 刘国臣 陈瑞兰 曹瑞 

机构地区：[1]河北省围场县动物疫病预防控制中心,河北围场068450 [2]青岛瑞尔生物技术有限公司,山东青岛266100

出　　处：《中国动物检疫》2016年第11期86-88,共3页China Animal Health Inspection

摘　　要：采用常规皮内变态反应(TST)、牛全血干扰素试验方法(IFN-γ)及胶体金3种检测方法,对2个奶牛场的600头奶牛进行结核病检测,发现结核病阳性率均偏高,表明奶牛场结核病感染较严重。将3种方法检测结果的敏感性、特异性及符合率进行比较,结果表明:单独使用PPD皮内变态反应方法或胶体金方法在牛结核病的诊断方面存在特异性及符合率差的缺点;用IFN-γ实验进行检测,可以提高特异性,避免因假阳性造成的误杀,适合在本地区推广应用。In order to compare the detection results of cow tuberculosis （TB） by different test methods, the tuberculin skin test （TST）, the whole blood interferon-gamma （IFN-y ） test and colloidal gold detection were applied in detecting TB of 600 cows from two farms. Results showed that the positive rates of TB by the three methods were all on the high side, which indicated the infection of TB in the cow farms was serious. Comparisons were made in aspects of sensitivity, specificity and coincidence rates of the testing results by the three methods. The results showed that the sensitivity and specificity were deficient when only using PPD intradermal allergy or colloidal gold to detect cow TB, and the specificity could be improved by the IFN- y test, avoiding manslaughter of cows due to false positive results, hence the IFN- y test was suitable to be applied in this area.

关 键 词：奶牛结核病 皮内变态反应试验 Γ-干扰素 胶体金 

分 类 号：S855.2[农业科学—临床兽医学]