苦参碱及氧化苦参碱对番茄灰霉菌的抑制效果  

Inhibition Effect Against Botrytis cinerea with Matrine and Oxymatrine

作　　者：姚红丽[1] 张浩[1] 叶嘉[1] 

机构地区：[1]邯郸学院生命科学与工程学院,河北邯郸056005

出　　处：《黑龙江农业科学》2016年第11期72-76,共5页Heilongjiang Agricultural Sciences

基　　金：河北省平台建设资助项目;邯郸学院校级资助项目(15105;15217)

摘　　要：为研究苦参碱及氧化苦参碱对番茄灰霉菌菌丝体和孢子的抑制作用,以番茄灰霉菌(Botrytis cinerea野生型菌株38B1)为供试靶菌,采用平皿法测定了不同浓度苦参碱(Matrine)和氧化苦参碱(Oxymatrine)对灰霉菌孢子萌发及菌丝体生长的抑制效果。结果表明:接菌培养时间相同,苦参碱和氧化苦参碱对灰霉菌丝体生长的最佳抑制浓度为8和10mg·mL-1。处理8h后,苦参碱浓度为3mg·mL-1、氧化苦参碱为4mg·mL-1时即能抑制孢子萌发。苦参碱及氧化苦参碱浓度、处理时间相同时,孢子悬浮液的滴加体积为0.06mL,孢子萌发率最低。In order to research the inhibition effect of matrine and oxymatrine against Botrytis cinerea,the plate method was adopted to observe inhibition effect that different concentrations of matrine and oxymatrine inhibi- ted spore germination and mycelial growth of Botrytis cinerea （wild type strain 38B1 ）. The results showed that Botrytis cinerea mycelial growth was completely inhibited by matrine of 8 mg ·mL^-1 and oxymatrine of 10 mg·mL^-1, After 8 h,when the matrine concentration was 3 mg·mL^-1 and the oxymatrine concentration was 4 mg·mL^-1 ,Botrytis cinerea spore germination was effectively inhibited. With the same concentrations of ma- trine and oxymatrine and treatment time adding the spore suspension 0.06 mL,Botrytis cinerea spores germi nation rate was the lowest.

关 键 词：苦参碱 氧化苦参碱 灰霉菌 菌丝体 孢子萌发 

分 类 号：S432.4[农业科学—植物病理学]