间歇生物接触氧化工艺中DOM三维荧光光谱分析  

Analysis of DOM by Excitation-emission Matrix Fluorescence Spectroscopy in Intermittent Bio-contact Oxidation

作　　者：张华[1,2] 杨程越 黄健[1,2] 田纪宇[1,2] 田丰 刘航[1,2] 周建宇[1,2] 凌玲[1,2] 

机构地区：[1]安徽省水污染控制与废水资源化安徽省重点实验室 [2]安徽建筑大学环境与能源工程学院,安徽合肥230601 [3]安徽长之源环境工程有限公司,安徽合肥230036

出　　处：《环境科学与技术》2016年第10期87-91,122,共6页Environmental Science & Technology

基　　金：国家水体污染控制与治理科技重大专项(2014ZX07303-003-09;2014ZX07405-003-03);安徽高校省级自然科学项目(KJ2013B049)

摘　　要：采用三维荧光光谱技术研究间歇生物接触氧化工艺处理城市污水的反应过程中溶解性有机物(DOM)的降解特性及其变化规律,并建立DOM总荧光强度与COD浓度的关系。三维荧光光谱显示:DOM主要由低激发波长色氨酸特征峰(峰A)和高激发波长色氨酸特征峰(峰B)及可见光区富里酸特征峰(峰C)组成。峰A和峰B的荧光强度均在曝气阶段逐渐降低而在停止曝气阶段逐渐增强,峰C的荧光强度在整个反应周期内变化不大,揭示了色氨酸在曝气阶段逐渐被微生物降解,在停止曝气阶段被吸附的大分子有机物分解而增加提供了此阶段反硝化脱氮所需的碳源,而类富里酸属于难降解有机物。荧光指数(f_(450/500))、生物源指数(BIX)和腐殖化指数(HIX)平均值分别为1.75、1.05和1.83,均表明工艺中DOM主要由微生物代谢而产生。DOM总荧光强度与COD浓度关系表明,在曝气、停止曝气阶段分别具有较好的相关性,相关系数R^2分别达到0.966、0.947,说明通过三维荧光光谱技术检测DOM特征峰荧光强度可以快速分析COD浓度变化,为污水处理实时监测及避免二次污染提供技术理论支持。This study probes the composition and transformation of dissolved organic matter（DOM） using excitation-emission matrix spectroscopy, and investigates the correlations between the total fluorescence intensity and COD concentration in sewage treatment process with intermittent bio-contact oxidation. The fluorescence spectra reveals three types of characteristic fluorescence peaks, i.e. tryptophan fluorescence peaks at high excitation wavelength（peak A）, tryptophan fluorescence peaks at high excitation wavelength（peak B）, and visible fulvic acid fluorescence peaks（peak C）. The fluorescence intensities of peak A and peak B decrease under the aeration condition, but increase during non-aeration, while that of peak C are not noticeable, implying a larger degradation of tryptophan during aeration and an obviously increase due to the decomposition of macromolecular organic matter, whereas fulvic acids are difficult to be degraded by microorganisms.The average value of fluorescence index（f（450/500））, biogenic index（BIX） and humification index（HIX） are 1.75, 1.05 and 1.83,total fluorescence intensities of DOM shows good linear correlations with COD concentration under aeration and non-aeration respectively, implying DOM is mainly produced by microbial metabolism in intermittent bio-contact oxidation. Under nonaeration conditions, the correlation coefficients are 0.966 and 0.947 respectively. The study proves that COD concentration can be estimated rapidly by measuring the total fluorescence intensity of DOM with excitation-emission matrix spectroscopy, and providing theoretical support for real time monitoring and avoiding secondary pollution in sewage treatment.

关 键 词：间歇生物接触氧化 三维荧光光谱 溶解性有机物 荧光强度 COD 

分 类 号：X832[环境科学与工程—环境工程]