清化颗粒对db/db糖尿病小鼠肠道环磷腺苷和三磷酸腺苷的影响  

Effects of Qinghua Granule on cAMP and ATP in Ileum Tissue of db/db Mice

作　　者：李俊燕[1] 王静懿[1] 徐杰[1] 陆灏[1] 陶枫[1] 

机构地区：[1]上海中医药大学附属曙光医院糖尿病研究所内分泌科,上海201203

出　　处：《辽宁中医杂志》2016年第11期2398-2400,I0003,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81403359);中国博士后科学基金(2015M581653);上海市中医药事业发展三年行动计划项目(ZY3-CCCX-2-1003);上海市自然科学基金(13ZR1442700)

摘　　要：目的:探讨清化颗粒对db/db糖尿病小鼠肠道环磷酸腺苷(c AMP)和三磷酸腺苷(ATP)的影响。方法:6只雄性db/m小鼠为空白对照组,24只雄性db/db糖尿病小鼠随机分为模型对照组、清化颗粒低剂量组、清化颗粒中剂量组和清化颗粒高剂量组,干预4周,IPGTT法观察小鼠血糖水平变化,ELISA法分析小鼠肠道c AMP、ATP和血清胰高血糖素样肽-1(GLP-1)的水平,组织病理学观察小鼠回肠组织形态变化。结果:与模型对照组比较,清化颗粒下调db/db糖尿病小鼠的空腹血糖(FBG)(P<0.01),增加肠道c AMP水平(P<0.01),降低肠道ATP水平(P<0.01)和血清GLP-1水平(P<0.01),并具有剂量依赖性。清化颗粒明显改善db/db糖尿病小鼠回肠组织的形态。结论:清化颗粒能够降低db/db糖尿病小鼠的血糖水平,促进GLP-1分泌,改善回肠组织形态,其机制可能与增加肠道c AMP水平,降低肠道组织中ATP水平有关。Objective：To explore the intervention effect of Qinghua Granule （QHG） on ATP and cAMP in db/db mice ileum tissue. Methods ： Six male db/m mice were as normal control group. Twenty - four male db/db mice were randomized into model control group, QHG low - dose group, QHG middle - dose group and QHG high - dose group. All the groups were administrated for 4 weeks. Blood glucose was detected by IPGTT. The serum concentration of GLP - 1 was tested by ELISA. The levels of ATP and cAMP in ileum of db/db mice were detected by ELISA. The structure of ileum tissue was observed by histopathological analysis. Results：Compared with model control group, QHG could decrease FBG and increase GLP - 1 secretion obviously （P 〈 0. 01 ）. QHG downregulated the cAMP levels and up- regulated the ATP level in ileum tissue significantly （P 〈 0. 01 ）. In addition, QHG obviously improved the structure of ileum of db/db mice. Conclusion ： QHG could reduce FBG obviously, improve GLP - 1 secretion and the structure of ileum and the mechanism might be related to the regulation of ATP and cAMP.

关 键 词：清化颗粒 DB/DB糖尿病小鼠 胰高血糖素样肽-1 三磷酸腺苷 环磷酸腺苷 

分 类 号：R285[医药卫生—中药学]