真菌诱导子对龙眼胚性愈伤组织生长和多糖积累的影响  

Effects of Fungal Elicitors on Growth and Polysaccharide Accumulation of Embryogenic Calli in Dimocarpus longan Lour.

作　　者：徐小萍[1] 赖瑞联[1] 林玉玲[1] 赖钟雄[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002

出　　处：《热带作物学报》2016年第11期2216-2221,共6页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金(No.31572088;No.31272149);福建省重大科技专项(No.2015NZ-0002-1)

摘　　要：为探讨真菌诱导子对龙眼胚性愈伤组织生长及多糖含量的影响。以龙眼胚性愈伤组织为材料,采用单因素分析法探究了真菌诱导子种类、诱导时间和浓度对龙眼胚性愈伤组织生长和多糖积累的影响。结果表明,不同真菌诱导子处理下龙眼胚性愈伤组织中多糖含量由高到低排序分别为:龙眼拟茎点霉>胶孢镰刀菌>尖孢镰刀菌;采用100 mg/L浓度的龙眼拟茎点霉诱导子诱导15 d,龙眼胚性愈伤组织多糖含量最高,比对照提高了61.34%,且不同处理间存在显著差异。但真菌诱导子处理下龙眼胚性愈伤组织生长状态较差,且增殖率显著下降。龙眼拟茎点霉作为龙眼病原内生菌,其活性诱导物能促进龙眼胚性愈伤组织中多糖积累,从而一定程度上提高植物的免疫防御能力。研究结果为探究真菌诱导子对龙眼多糖合成和代谢机理的研究奠定基础。In this experiment, the embryogenic calli were used as the materials to investigate the influence of the types, induction time, and concentrations of fungal elicitors for the growth and the accumulation of polysaccharide in longan embryogenic calli by single factor analysis in Dimocarpus longan Lour. The results showed that the order of the polysaccharide contents under different fungal elicitors was： Phomopsis longanae Chi〉 Fusarium subglutinans Wollenw.et Reinking 〉Fusarium oxysporum. Treated by 100 mg/L Phomopsis longanae Chi elicitor for15 d, the accumulation of polysaccharide reached the highest level. Moreover, there were significant differences between different treatments. However, the proliferation and growth situation was worse than that of the control.Studies suggested that P. longanae Chi was longan fruit pathogenic endophyte, the active inducer of P. longanae Chi could observably promote longan polysaccharide accumulation in embryonic callus and improve the immune defense ability of plants. The above results could provide the scientific basis for studying longan polysaccharide synthesis and metabolic mechanism.

关 键 词：龙眼 真菌诱导子 多糖 胚性愈伤组织 增殖 

分 类 号：S667.2[农业科学—果树学]