准噶尔盆地南缘和阿拉善左旗9个梭梭居群种间系统发育和亲缘关系研究  

Phylogeny and genetic relationship of 9 Haloxylon ammodendron populations in Junggar basin and Alxa Left Banner

作　　者：高志娟[1] 庄丽[1] 

机构地区：[1]石河子大学生命科学学院,石河子832000

出　　处：《干旱区资源与环境》2016年第11期186-190,共5页Journal of Arid Land Resources and Environment

基　　金：国家重点基础研究发展计划(973计划)亚欧内陆荒漠生态系统对全球变化的响应特征与区域生态安全(2014CB954203);国家自然科学基金地区项目(31360139)资助

摘　　要：对荒漠植物梭梭进行ITS序列测定,研究不同梭梭居群系统发育和亲缘关系。材料源于准噶尔盆地南缘及内蒙古阿拉善左旗。结果表明:所测序列经排序后全长732bp。遗传距离矩阵表明:阜康与阿左旗的梭梭居群间遗传距离最小;石河子150与富蕴的居群遗传距离最大。系统发育树结果显示:石河子121、克拉玛依、杜热和150的居群聚为一支;阜康与阿左旗居群以92%的自展支持率相聚;戈壁藜等三种植物作为外类群。In order to compare the phylogeny and genetic relationships of different Haloxylon ammodendron populations,ITS sequences were measured and analyzed. We selected 9 populations which were gotten from Jungar Basin and the Alxa Left Banner located in southwestern Inner Mongolia. The results showed： the whole length of ITS sequence was 732 bp after aligned and cut. Genetic distance matrix indicated that the genetic distance between the populations of Fukang and Inner Mongolia Azuoqi had the minimum value 0. 001,which showed their phylogenetic relationship was the closest. While the maximum value was 0. 565 which existed between the populations of Shihezi 150 and Fuyun,which showed their phylogenetic relationship was the farthest. The molecular phylogenetic trees suggested that the populations of Haloxylon ammodendron in Shihezi 121,150,Karamay and Dure were gathered for a branch,which showed the four populations had highly close phylogenetic relationship.The two populations of Fukang and Inner Mongolia Azuoqi gathered alone with 92% bootstrapping approval rating. Three kinds of Chenopodiaceae plants were located outside the phylogenetic tree as outgroup,such as Iljinia regelii.

关 键 词：梭梭 ITS序列 聚类分析 遗传距离矩阵 系统发育树 

分 类 号：Q945[生物学—植物学]