机构地区:[1]National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032,China [2]College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China [3]University of Chinese Academy of Sciences, 19A Yuquan Road,Beijing 100049, China [4]Department of Plant and Microbial Biology, Ztirich-Basel Plant Science Center, University of Zurich, 8008 Zurich, Switzerland
出 处:《Science Bulletin》2016年第22期1728-1731,共4页科学通报(英文版)
基 金:supported by the National Basic Research Program of China (973 Program,2014CB943500/2012CB910503);the National Natural Science Foundation of China (91419302/31422005);Youth Innovation Promotion Association CAS
摘 要:Many differentiated plant organs have the ability to regenerate into a new plant after detachment via de novo organogenesis. During de novo root organogenesis from Arabidopsis thaliana leaf explants, wounding first induces endogenous auxin production in mesophyll cells. Auxin is then polar transported to, and accumulates in, regenerationcompetent cells near the wound to trigger the cell-fate transition. The TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS(TAA) family proteins and the YUCCA(YUC) family proteins catalyze two successive biochemical steps in auxin biogenesis, and YUCs have been shown to be involved in auxin production in mesophyll cells during de novo root organogenesis. In thisstudy, we show that the TAA family is also required for adventitious rooting. Inhibition of TAA blocked adventitious root formation from leaf explants. Intriguingly,whereas YUC1 and YUC4 have been shown to be highly induced by wounding, TAA genes retained consistent expression levels before and after leaf detachment.Therefore, we suggest that TAAs and YUCs are both required for auxin biogenesis in leaf explants, but they play different roles in regeneration. While YUC1 and YUC4 function in response to wounding to catalyze the ratelimiting step in auxin biosynthesis, TAAs probably serve as abiding and basal enzymes during de novo root organogenesis from leaf explants.Many differentiated plant organs have the ability to regenerate into a new plant after detachment via de novo organogenesis. During de novo root organogenesis from Arabidopsis thaliana leaf explants, wounding first induces endogenous auxin production in mesophyll cells. Auxin is then polar transported to, and accumulates in, regeneration- competent cells near the wound to trigger the cell-fate transition. The TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS (TAA) family proteins and the YUCCA (YUC) family proteins catalyze two successive biochemical steps in auxin biogenesis, and YUCs have been shown to be involved in auxin production in meso- phyll cells during de novo root organogenesis. In this study, we show that the TAA family is also required for adventitious rooting. Inhibition of TAA blocked adventi- tious root formation from leaf explants. Intriguingly, whereas YUC1 and YUC4 have been shown to be highly induced by wounding, TAA genes retained consistent expression levels before and after leaf detachment. Therefore, we suggest that TAAs and YUCs are both required for auxin biogenesis in leaf explants, but they play different roles in regeneration. While YUC1 and YUC4 function in response to wounding to catalyze the rate- limiting step in auxin biosynthesis, TAAs probably serve as abiding and basal enzymes during de novo root organo- genesis from leaf explants.
关 键 词:De novo root organogenesis TAA1TAR2 Plant regeneration Auxin biogenesis Arabidopsis
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...