抗菌肽LL-37及其衍生物对肝癌细胞HepG-2增殖和凋亡的影响效果比较  

Comparison of the Effects of LL-37 and Its Derivative on the Proliferation and Apoptosis of Hepatocellular Carcinoma HepG-2 Cell Line

作　　者：杨浩[1,2] 柯俊[1] 付靖瑜 汪宏良[1,2] 罗鹏程 

机构地区：[1]鄂东医疗集团黄石市中心医院(湖北理工学院附属医院)医学检验科,湖北黄石435000 [2]肾脏疾病发生与干预湖北省重点实验室,湖北黄石435000

出　　处：《现代生物医学进展》2016年第34期6622-6625,共4页Progress in Modern Biomedicine

基　　金：湖北省自然科学基金项目(2015CFB627);黄石市科技计划项目(2013A077-4)

摘　　要：目的:比较抗菌肽LL-37及其衍生物对肝癌细胞HepG-2增殖和凋亡的影响。方法:以肝癌细胞HepG-2为靶细胞,采用不同浓度抗菌肽LL-37及其衍生物处理后,通过四甲基偶氮噻唑蓝(MTT)比色法检测细胞的增殖情况,流式细胞术Annexin V/PI双染色测定细胞的凋亡情况。结果:随着抗菌肽LL-37及其衍生物作用浓度的增加和作用时间的延长,其对肝癌细胞增殖的抑制作用逐渐增强,二者的浓度都达到300μg/m L时作用72 h,对肝癌细胞增殖的抑制作用最大(P<0.01),且抗菌肽LL-37衍生物对肝癌细胞增殖的抑制率高于抗菌肽LL-37。流式检测结果显示:抗菌肽LL-37衍生物诱导的细胞凋亡率高于抗菌肽LL-37。结论:抗菌肽LL-37衍生物在抗肝癌细胞增殖和促进其凋亡方面的作用优于抗菌肽LL-37。Objective： To compare the effect of peptide LL-37 and its derivative on the proliferation and apoptosis of hepatocellular carcinoma HepG-2 cell line. Methods： The hepatocellular HepG-2 carcinoma cell line was used as targeted cells. MTT assay was applied to detect the effects of different concentrations of LL-37 peptide and its derivative on the proliferation of HepG-2 cells. And the flow cytometry annexin V/PI double staining was used to determine the cell apoptosis. Results： With the increase of the concentrations of LL-37 peptide and its derivative and the exposed time, the inhibitory rates on the proliferation of HepG-2 cells were gradually increased. The highest inhibitory rate occurred by LL-37 and its derivative with the concentration of 300 μg/mL after 72 h expose, and peptide LL-37 derivative induced a higher inhibitory rate on the proliferation of HepG-2 cells than that ofPeptide LL-37. The results of flow cytometry showed that the apoptosis rate induced by peptide LL-37 derivative was higher than that of peptide LL-37. Conclusion： Peptide LL-37 derivative could better enhance the apoptosis and inhibit the proliferation of HepG-2 cells than peptide LL-37.

关 键 词：抗菌肽LL-37 衍生物 细胞凋亡 肝癌细胞 

分 类 号：R33[医药卫生—人体生理学]