大麻素抑制背根节神经元ATP诱发的[Ca^(2+)]i升高  

Cannabinoid inhibit ATP- induced [Ca^(2+)]i increase in the dorsal root ganglion neurons

作　　者：孙涛[1] 陈美云[1] 雷晓露[1] 曾俊伟[1] 陈远寿[1] 余德芊[1] 田虹[1] 刘晓红[1] 

机构地区：[1]遵义医学院基础医学院生理学教研室,遵义563000

出　　处：《神经解剖学杂志》2016年第6期684-690,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(31360253);贵州省科学技术基金项目(黔科合J[20082314);遵义医学院重点学科建设项目(NO:0996032)

摘　　要：目的:观察大麻素对背根节神经元ATP诱发的[Ca^(2+)]i升高的影响及机制。方法:培养SD大鼠背根节神经元,采用激光共聚焦技术检测培养神经元[Ca^(2+)]i的变化。结果:ATP(100μmol/L)经P2X受体介导可导致培养的背根节神经元[Ca^(2+)]i增高(P<0.05);大麻素受体激动剂CP55940预孵育10 min可剂量依赖性地抑制背根节神经元ATP所致的[Ca^(2+)]i升高(P<0.05);CB1受体(cannabinoid receptor 1,B1R)的拮抗剂AM251(10μmol/L)、CB2受体(Cannabinoid receptor 2,CB2R)的拮抗剂AM630(10μmol/L)均可显著降低CP55940(1μmol/L)的抑制效应(P<0.05);腺苷酸环化酶激动剂Forskolin(10μmol/L)可逆转CP55940对ATP的抑制作用(P<0.05)。结论:CP55940可显著抑制背根节神经元ATP诱发的[Ca^(2+)]i升高,CP55940的抑制效应可能是由CB1、CB2受体介导抑制背根节神经元PKA活性所致。Objective： To explore whether cannabinoid has an effect on ATP-induced intracellular calcium concentration [ Ca2＋ ] i increase in cultured dorsal root ganglion neurons and the related mechanism. Methods： Neurons from SD rat dorsal root ganglion were cultured. Changes of intracellular calcium fluorescence intensity in cultured DRG neurons were measured by laser scanning confocal microscopy. Results： ATP （100 μmol/L） caused the increase of [ Ca2＋] i in DRG neurons via activation of P2X receptors （ P 〈 0.05 ）. A 10 min incubation with CP55940, the agonist of cannabinoid receptor, inhibited ATP-induced [ Ca2＋ ]i increase in DRG neurons in a concentration-dependent manner. The inhibitory effect of CP55940 on ATP-induced [ Ca2＋] i increase was blocked by CB1 receptor （ Cannabinoid receptor 1, CB1R） antagonist AM251 （ 10 μmol/L） and CB2 receptor （ Cannabinoid receptor 2, CB2R） antagonist AM630 （ 10 μmol/L）, respectively（P 〈 0.05）. Forskolin （adenosine cyclase agonist, 10 μmol/L） markedly suppressed the inhibitory effect of CP55940 （P 〈 0.05 ）. Conclusion： CP55940 can inhibit ATP-induced [ Ca2＋] i increase in DRG neurons. The inhibitory effect of CP55940 may be mediated by CB1 and CB2receptors, which block PKA activity of DRG neurons.

关 键 词：大麻素 背根节神经元 P2X受体 CA^2+ 大鼠 

分 类 号：R285[医药卫生—中药学]