七氟醚处理对老龄小鼠基底前脑胆碱能系统和认知功能的影响  

Effect of sevoflurane on basal forebrain cholinergic system and cognition in aged mice

作　　者：熊璐 徐文庆 王子高[2] 

机构地区：[1]上海市金山区亭林医院麻醉科,上海201505 [2]复旦大学附属金山医院神经内科,上海201508

出　　处：《神经解剖学杂志》2016年第6期757-762,共6页Chinese Journal of Neuroanatomy

基　　金：上海市卫生和计划生育委员会青年项目(20165Y0193);上海市金山区卫生和计划生育委员会青年项目(JSKJ-KTQN-2015-16;SKJ-KTQN-2014-08)

摘　　要：目的:探讨七氟醚对基底前脑胆碱能系统和认知功能的影响。方法:老年C57小鼠吸入七氟醚建立全身麻醉动物模型,利用Morris水迷宫检测认知功能,免疫荧光检测Meynert基底核中胆碱能神经元计数,Western Blot检测额叶皮层胆碱乙酰转移酶及高亲和力胆碱转运体的蛋白水平,ELISA测定额叶皮层胆碱乙酰转移酶的活性。结果:与对照组相比,七氟醚组小鼠平台潜伏期延长,平台穿越次数减少(2.1±0.4 vs 5.6±0.5,P<0.05),原平台所在象限停留时间缩短[(21.5±2.4)%vs(48.6±2.8)%,P<0.05]。此外,七氟醚组小鼠Meynert基底核胆碱能神经元计数减少(3748±248 vs 5942±315,P<0.05),额叶皮层胆碱乙酰转移酶及高亲和力胆碱转运体表达下降,伴有胆碱乙酰转移酶活性降低[(31.4±1.1)μmol/(g.h)vs(55.8±1.3)μmol/(g.h),P<0.05]。结论:七氟醚可损害老龄鼠基底前脑胆碱能系统并诱导认知功能损害。Objective： To explore the effect of sevoflurane on basal forebrain cholinergic system and cognition. Methods： Aged C57 mice were given sevoflurane to induce model of general anesthesia. Cognition was assessed by Morris water maze. Cholinergic neurons in nucleus basalis of Meynert were evaluated by immunofluorescence. Protein levels of choline acetyltransferase and high affinity choline transporter in frontal cortex were determined by Western Blot. Enzyme activity of choline acetyltransferase in frontal cortex was measured by ELISA. Results： Compared with controls, mice treated with sevoflurane showed longer escape latency, less platform crossing （2.1 ±0. 4 vs 5.6 ±0. 5, P 〈 0. 05 ） and shorter time spent in target quadrant [ （21.5 ± 2.4） % vs （48.6 ± 2.8） %, P 〈 0.05 ]. In addition, mice treated with sevoflurane exhibited loss of cholinergic neurons in nucleus basalis of Meynert （3748 ± 248 vs 5942 ± 315, P 〈0. 05 ）, decreased expression of choline acetyhransferase and high affinity choline transporter, as well as inhibited enzyme activity of choline acetyltransferase [ （ 31.4 ± 1.1 ） μmoL/（ g. h） vs （ 55.8 ± 1.3 ） μmol/（ g. h ）, P 〈0. 05 ]. Conclusion： Sevoflurane could lead to impairment of basal forebrain cholinergic system and cognitive dysfunction in aged mice.

关 键 词：七氟醚 基底前脑胆碱能系统 认知功能 MORRIS水迷宫 胆碱乙酰转移酶 小鼠 

分 类 号：R614[医药卫生—麻醉学]