珍稀树种花榈木种子休眠破除方法研究  

Study on Method of Breaking Seed Dormancy for Rare Tree Species Ormosia henryi

作　　者：邓兆[1,2] 韦小丽[1] 

机构地区：[1]贵州大学林学院,贵阳550025 [2]长顺县林业局,贵州长顺550700

出　　处：《种子》2016年第11期1-4,共4页Seed

基　　金：国家自然科学基金"花榈木根瘤菌多样性及幼苗形成调控因素研究"(编号:31460193);国际合作项目"珍贵用材树种花榈木精准化容器育苗理论与技术研究"(编号:黔科合外G字[2012]7012号);贵州省林业厅重大攻关项目"贵州优质阔叶用材树种培育技术与示范"(编号:黔林科合[2010]重大02)

摘　　要：为了破除花榈木种子的休眠,对花榈木种子进行药剂处理、混沙湿藏处理以及激素+混沙湿藏处理,对处理后的种子进行发芽试验,测定种子的发芽指标,并测定混沙湿藏不同阶段种子内部脱落酸、赤霉素、酸性磷酸酯酶、淀粉酶、蛋白酶、ATP等指标的变化。结果表明:激素+混沙湿藏处理的种子萌发效果优于混沙湿藏和药剂处理。用500mg/L赤霉素浸泡花榈木种子12h,再低温(4℃)混沙湿藏45d是破除花榈木种子休眠的最佳方法,其发芽开始时间、结束时间和高峰期分别比不处理的种子提早10,17d和14d,发芽率、发芽势分别比对照提高36.6%、32.7%。混沙湿藏处理的花榈木种子的酸性磷酸酯酶、淀粉酶和蛋白酶活性、GA3和ATP含量随着混沙湿藏的时间延长而增加,ABA含量则随着混沙湿藏时间的延长而减小,混沙湿藏对破除花榈木种子休眠起关键作用。For breaking seed dormancy of Ormosia henryi,the Ormosia henryi seed were treated by medicament,stratification,hormone combine with stratification.The germination experiment of seed after treatment were determined,and the change of ABA,GA3,acid phosphatase,diastase,protease and ATP of seed were determined at different stage after stratification.The results showed the germinate effect of seed by handing with hormone combine with stratification was better than stratification and medicament.The method that soaked seed for 12 husing 500mg/L GA3,then stratification for45 dunder 4℃ was the best method for breaking dormancy of Ormosia henryi seed,and the begin time,end time,peak time of germination ahead of 10 d,17dand 14 dthan control,the germination rate,germination potential improved 36.6% and 32.7%.The acid phosphatase,diastase and protease activity,GA3 and ATP content of Ormosia henryi seed increased with the time of stratification;and the ABA content decreased;the stratification played a key role for breaking the dormancy of Ormosia henryi seed.

关 键 词：花榈木 种子休眠 药剂处理 混沙湿藏 激素处理 

分 类 号：Q945.79[生物学—植物学]