马骨关节炎模型关节液中COMP含量变化的研究  

Changes in synovial fluid COMP from equine with experimentally-induced osteoarthritis

作　　者：李玥[1] 王冠颖[1] 李欣然[1] 姜仁礼 范晓静[1] 刘旭[1] 王晶璐 高利[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2016年第10期12-13,16,共3页Chinese Journal of Veterinary Medicine

基　　金：国家科技支撑计划资助(2012BAD46B02-03)

摘　　要：为探索马骨关节炎病变程度与关节液中软骨代谢标志物软骨寡聚基质蛋白(COMP)水平的关系,试验选用13匹蒙古马,随机分为试验组(n=8)和对照组(n=5)试验组左侧腕关节内注入2 mL两性霉素B(25 mg/mL体重),建立马骨关节炎模型;对照组左侧腕关节注射2 mL生理盐,水。每周采集关节液1次,用ELISA法检测马关节液中COMP的含量。结果显示,试验组关节液COMP浓度在注射两性霉素B后第14天开始显著升高(15.54μg/L±1.46μg/L),与对照组(11.29μg/L±0.73μg/L)比较差异极显著(P<0.01);试验组COMP浓度保持增高的趋势直至第63天试验结束;第21天试验组马匹开始出现不同程度跛行。试验结果表明,关节液内COMP浓度升高比马跛行出现得早,推测COMP可以作为马骨关节炎(OA)早期诊断的生物标记物。To study the relationship of lesion extent of osteoarthritis to COMP level,the marker of cartilage metabolites,in synovial fluid,13 horses were divided into experimental（n =8） and control groups（n =5）,Acute osteoarthritis was induced in 8 horses by a sterile intra-articular injection of 2 mL Amphotericin-B（25 mg/mL）into the left carpal joint,and the control group was injected into the left carpal joint with 2ml Saline.Synovial fluid was collected once a week,and the concentrations of COMP were measured with ELISA kit.Compared with the control group（11.29μg/L ±0.73 μg/L）,the level of COMP in synovial fluid was significantly increased in the experimental group at the 14 th day（15.54 μg/L ± 1.46μg/h）,and lameness appeared at the 21 th day.The results demonstrated that the level of COMP in synovial fluid increased earlier than die lameness appearance,and the COMP could be used as a biomarker for early diagnosis of equine osteoarthritis.

关 键 词：骨关节炎 两性霉素B COMP 

分 类 号：S821.81[农业科学—畜牧学] S857.165[农业科学—畜牧兽医]