3种脂血消除法在生化测定中的对比研究  

Comparative Study of 3 Kinds of Blood Fat Elimination Method in Biochemical Measurement

作　　者：陈燕[1] 

机构地区：[1]云南省个旧市红河州第三人民医院检验科,云南个旧661000

出　　处：《中外医疗》2016年第31期27-29,共3页China & Foreign Medical Treatment

摘　　要：目的研究比较3种不同方法消除脂血对生化测定结果干扰的效果。方法方便选取2015年4月—2016年4月该院收治的76例患者的脂血标本,将标本均分为四等份,用四种方法进行生化测定,对其结果进行分析比较。结果K+指标四组之间生化测定值(4.1±0.2)、(4.1±0.1)、(4.2±0.1)、(4.2±0.3),差异无统计学意义(P>0.05);处理组(离子水稀释组、高速离心组和乙醚萃取组)Ca2+、TP等指标的生化测定结果与未处理组(原血浆组)对比,差异有统计学意义(P<0.05),原血浆测定值最高,与原血浆相比较,乙醚萃取法处理后的标本测定值降低第最为显著,高速离心法处理后的标本测定值降低次之,离子水稀释法降低幅度最小。结论脂血可能会使得生化测定结果中Ca2+、TP等指标测定值偏大;三种消除脂血对蛋白质类的生化测试影响由强到弱依次为乙醚萃取法、高速离心法、离子水稀释法。在临床工作中可根据检测目的以及具体设备配置的不同进行使用。Objective To study three methods of removing the distrubing effect of lipemia in the biochemical measurement results. Methods Convenient selected by April 2015 to April 2016, 76 patients of fat blood samples, the samples were divided into four equal parts, with four methods of biochemical determination. The results were compared. Results There were no significant differences in biochemical parameters between the four groups（4.1 ±0.2）,（4.1±0.1）,（4.2±0.1）,（4.2±0.3）, and there was no statistical significance（P〈0.05）. after measurements, the index of Ca2＋,TPfor four groups was significantly different（P〈0.05）; The index ranged from high to low：plasma detection, ultracentrifugation method,dilution method of ionized water and aether extraction method. Conclusion The methods of removing the distrubing effect of lipemia can improve the detection accuracy of biochemical measurements.The clinical effect of three methods ranges from high to low： aether extraction method,dilution method of ionized water, ultracentrifugation method.

关 键 词：高血脂症 生化测定 高速离心法 

分 类 号：R28[医药卫生—中药学]