超临界CO_2与乙醇结合工艺制备蛋黄油及其质量控制  

Preparation of Egg Yolk Oil by supercritical CO_2 and Ethanol Combined Process and Its Quality Control

作　　者：林影影 阳向波 纪奇森 韩丽[1] 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室四川省中药资源系统研究与开发利用重点实验室--省部共建国家重点实验室培育基地,四川成都611137 [2]成都百草和济科技有限公司,四川成都610000 [3]四川联成迅康医药股份有限公司,四川成都610000

出　　处：《亚太传统医药》2016年第23期28-31,共4页Asia-Pacific Traditional Medicine

摘　　要：目的：研究超临界CO2与乙醇结合工艺提取蛋黄油，并建立其质量控制方法。方法：分别采用TLC法鉴别蛋黄油中β谷甾醇和卵磷脂成分；HPLCELSD法测定蛋黄油中磷脂酰胆碱的含量；紫外-可见分光光度法对蛋黄油中总磷量进行测定。结果：超临界CO2与乙醇结合工艺制备的蛋黄油性状稳定；TLC均能检测出蛋黄油中β-谷甾醇和卵磷脂的特征斑点；HPLC-ELSD法精密度、重现性良好，磷脂酰胆碱在50．49～400．72μg／mL范围内均有较好的线性关系，平均加样回收率为97．68％，RSD为1．75％；紫外-可见分光光度法测得的线性关系良好，平均回收率为100．63％，RSD为2．67％。结论：该方法操作简单，工艺良好，为建立和完善蛋黄油质量标准提供参考依据。Objective：To study the preparation of egg yolk oil by supercritical CO2 and ethanol extraction process, and to establish the quality control method. Methods： TLC identification method of yolk oil beta sitosterol and lecithin ingredients; HPLC ELSD method for the determination of content of phosphatidylcholine from egg yolk oil; ultraviolet visible speetropbotometry was used to determine the amount of total phosphorus in egg yolk oil. Results： Supercritieal CO2 and ethanol binding process in the preparation of egg yolk oil steady state stability is good; TLC could be detected in all the characteristic spots of eggs in butter beta sitosterol and lecithin; HPLC-ELSD method precision, reproduce well, phosphatidyl choline in 50.49 to 400.72μg/mL are a good linear relation- ship, with the average recovery rate was 97.68 %, RSD was 1.75% ; ultraviolet visible spectrophotometry measuring the linear rela- tionship was good. The average recovery rate for 100.63 %, RSD 2. 67%. Conclusion：To provide reference for establishing and improving the quality standard of egg yolk oil.

关 键 词：蛋黄油 HPLC-ELSD 紫外可见分光光度法 磷脂酰胆碱 

分 类 号：R284[医药卫生—中药学]