云南汉族人群LMP基因多态性与丙型肝炎病毒慢性感染的相关性  

Association of LMP gene polymorphisms with chronic HCV infection among ethnic Han population from Yunnan

作　　者：李彤[1] 刘城秀 姚宇峰[2] 俞建昆[2] 史荔[2] 沈云松[1] 

机构地区：[1]云南省第一人民医院,昆明理工大学附属昆华医院,昆明650032 [2]中国医学科学院&北京协和医学院医学生物学研究所,云南省重大传染病疫苗研发重点实验室,昆明650118

出　　处：《中华医学遗传学杂志》2016年第6期806-810,共5页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金（81160197）;吴阶平医学基金（320.6750.13395）

摘　　要：目的探讨低分子量蛋白酶体（low molecular weight polypeptide, LMP）基因多态性与丙型肝炎病毒（hepatitis C virus , HCV）慢性感染的相关性。方法选择云南地区汉族人群HCV慢性感染患者427例，健康对照者412名，采用TaqMan探针基因分型方法对LMP2基因单核苷酸多态性（ single nucleotide polymorphism, SNP）rs1351383、rs17587、rs2127675和LMP7基因SNPrs2071543进行基因分型，并构建单倍型，统计LMP2和LMP7多态性位点的等位基因频率与基因型频率、单倍型频率，分析SNPs及单倍型与HCV慢性感染的相关性。结果病例组和对照组LMP2基因rs1351383和rs2127675的等位基因频率差异有统计学意义（P〈0．05），rs1351383／rs17587／rs2127675单倍型A-pA和C-G-G差异有统计学意义（P〈0．05）；其他位点基因型、等位基因型、单倍型频率在病例组和对照组中差异无统计学意义（P〉0．05）。结论在云南汉族群体中，LMP2基因SNPrs1351383C等位基因和rs2127675G等位基因可能是HCV慢性感染的易感因素，rs1351383／rs17587／rs2127675单倍型A-GA可能是HCV慢性感染的保护因素，单倍型C-G-G可能是HCV慢性感染的危险因素。Objective To assess the association of single nucleotide polymorphisms （SNPs） of the low molecular weight polypeptide （LMP） gene with chronic hepatitis C virus （HCV） infection among ethnic Han population from Yunnan. Methods A total of 427 patients with chronic HCV infection and 412 healthy controls were recruited. SNPs rs1351383, rs17587 and rs2127675 from the promoter region of the LMP2 gene and rs2071543 from the promoter region of the LPM7 gene were genotyped using a TaqMan probe. The haplotypes were constructed. Frequencies of various alleles, genotypes and haplotypes of the selected SNPs were calculated, and their association with chronic HCV infection was analyzed. Results The frequencies of rs1351383 and rs2127675 alleles of the LMP2 gene, as well as the A-G-A and C-C-G haplotypes of the rs1351383/rs17587/rs2t27675 loci, had differed significantly between the two groups （P〈0.05）. Conclusion The C allele of the rs1351383 locus and G allele of the rs2217675 locus of the LMP2 gene may be susceptible factors for chronic HCV infection among ethnic Han people from Yunnan. The A-G-A haplotype of the rs1351383/rs17587/rs2127675 loci may confer a protective effect, while the C- G-G haplotype may be a susceptible factor for chronic HCV infection in this population.

关 键 词：丙型肝炎病毒 慢性感染 单核苷酸多态性 LPM2基因 LMP7基因 

分 类 号：R512.63[医药卫生—内科学]