3种高山杜鹃对低温的生理响应  

Physiological Responses of Three Species of Rhododendron under Low Temperature

作　　者：黄承玲[1,2] 赵孝梨[1] 刘广超[2] 

机构地区：[1]贵州民族大学化学与环境科学学院,贵州贵阳550025 [2]贵州民族大学人文科技学院,贵州贵阳550025

出　　处：《西南农业学报》2016年第10期2346-2350,共5页Southwest China Journal of Agricultural Sciences

基　　金：贵州省科技厅国际科技合作项目"百里杜鹃杜鹃属植物种类与变异研究"[黔科合体外J字(2013)7035];贵州民族大学引进人才项目"高山杜鹃引种及应用示范研究"(15XRY011)

摘　　要：为探明高山杜鹃的抗寒性机理,促进其新品种选育及开发利用,通过人工控温方法研究低温对露珠杜鹃、大白杜鹃和迷人杜鹃叶片抗氧化酶活性及渗透调节物质、可溶性蛋白质和丙二醛(MDA)含量的影响。结果表明:随胁迫温度的降低,3种杜鹃叶片SOD活性呈下降后上升再下降趋势。其中,10℃时迷人杜鹃叶片SOD活性为对照的87.7%。大白杜鹃叶片POD活性呈上升趋势,0℃时POD活性为对照的1.58倍;迷人杜鹃和露珠杜鹃叶片POD活性呈先上升后下降趋势。3种杜鹃叶片渗透物质含量呈上升趋势。大白杜鹃和迷人杜鹃叶片的可溶性蛋白质含量呈先上升后下降趋势。露珠杜鹃叶片可溶性蛋白质的含量呈持续上升趋势。3种杜鹃叶片的MDA含量和质膜相对透性均呈上升趋势,且大白杜鹃的MDA含量和质膜相对透性较其他2种高,0℃时大白杜鹃、迷人杜鹃和露珠杜鹃叶片的MDA含量依次为18.97、12.5和9.97 nmol/mg。露珠杜鹃和迷人杜鹃的抗寒能力比大白杜鹃强。In order to find out the mechanism of cold resistance of Rhododendron and promote the development and utilization of new varieties, the activities of antioxidant enzymes, the content of osmotic adjustment substance, soluble protein and MDA of three Rhododendron species were measured through artificial temperature control. Results：with decreasing temperature, SOD activity of Rhododendron descend first, then ascend, and descend at last. At 10 ℃, SOD activity of R. agastum was 87.7 % of the control. POD activity of R. decorum raised constantly, POD activity was 1.58 times of the control at 0℃. But POD activity of R. agastum and R. irroratum increased first and then de- creased. The content of soluble sugar and proline increased, the soluble protein content of R. decorum and R. agastum ascend first, then descend last. However, the soluble protein of R. irroratum increased constantly. The relative membrane permeablility of them were enhanced constantly, and MDA content continually increased. MDA content and relative membrane permeability of R. decorum were higher than that of the other two species, MDA content of R. decorum was 18.97 nmol/mg at 0 ℃, MDA content of R. agastum and R. irroratum were 12.5 nmol/mg and 9.97 nmnl/mg respectively. The cold resistance of R. irroratum and R. delavayi were stronger than R. decorum.

关 键 词：高山杜鹃 低温 抗氧化酶活性 可溶性蛋白质含量 丙二醛含量 质膜相对透性 

分 类 号：S685.21[农业科学—观赏园艺] Q945.78[农业科学—园艺学]