香芹酚对STZ诱导的1型糖尿病小鼠糖脂代谢的影响  

Effect of CAR on STZ-induced type 1 diabetic mice

作　　者：麦云珮 张淑允[1] 窦纪梁 张贵平[1,2] 罗健东[1,2] 侯宁[1,2] MAI Yun-pei ZHANG Shu-yun DOU Ji-liang ZHANG Gui-ping LUO Jian-dong HOU Ning(Dept of Pharmacology, Guangzhou Medical University, Guangzhou 511436, China Guangzhou Institute of Cardiovascular Disease, Guangzhou Key Laboratory of Cardiovascular Disease, Guangzhou 510260, China Henan Vocational College of Applied Technology, Zhengzhou 450042, China)

机构地区：[1]广州医科大学药学院药理学教研室,广东广州511436 [2]广州心血管疾病研究所,广东广州510260 [3]河南应用技术职业学院,河南郑州450042

出　　处：《中国药理学通报》2016年第9期1306-1310,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金项目(No 81402928);广东省高等学校优秀青年教师培养计划(No YQ2015135);广州市教育科研计划项目(No 2012C199)

摘　　要：目的观察香芹酚对糖尿病小鼠糖脂代谢的影响。方法腹腔注射链脲佐菌素(streptozotocin,STZ)建立1型糖尿病C57BL/6小鼠模型,成模小鼠随机分成3组(糖尿病组、香芹酚10、20 mg·kg^(-1)低、高剂量组),以同周龄同性别正常小鼠为对照组。糖尿病组和对照组腹腔注射生理盐水,其余组每天腹腔注射香芹酚,每周测体重和随机血糖,分别在给药2、4、6周后处死,收集小鼠血清,检测血糖、血脂、血总胆固醇、肝功能指标。结果香芹酚可有效降低糖尿病小鼠的随机血糖、血脂和乳酸脱氢酶水平。结论香芹酚可降低1型糖尿病小鼠血糖、血脂,保护受损器官,对糖尿病及其并发症有一定的治疗作用。Aim Carvacrol（ CAR）,possesses a wide variety of pharmacological properties including antioxidant and anti-inflammatory potential. The present study is designed to investigate the effect of CAR on glucose and lipid metabolism in type 1 diabetic mice.Methods Diabetes was induced by intraperitoneal（ i.p） injection of streptozotocin into male mice at the dose of 45 mg·kg^（-1）body weight（ BW）. Mice were divided into three different groups containing eight to twelve in each. Age matched male C57 mice were used as normal controls. Group I diabetes,Group Ⅱ and Ⅱ injected with CAR at 10 and 20 mg · kg^（-1）BW respectively once daily. After CAR injection 2, 4 or 6weeks,the rats were weighted and the plasma concentrations of glucose,total cholesterol（ TC）,triglycerides（ TG）,Glutamic oxalacetic transaminase（ AST）,Alanine transaminase（ ALT） levels were enzymatically determined using commercial kits. Results STZ-induced C57 BL /6J diabetic mice showed an elevation in serum glucose,TG,ALT,AST and LDH levels. Compared to diabetic mice,administration of CAR resulted in significant decreases（ P 0. 05） in plasma glucose,TG and LDH levels in a dose dependent manner,but no effect on elevated TC,ALT and AST levels. Conclusion These major findings provide evidence that CAR has anti diabetic property and it has the potential for development into a drug to prevent hyperglycemia,reduce blood lipids and protect the dammaged organs.

关 键 词：香芹酚 糖尿病 C57BL/6小鼠 STZ 糖脂代谢 腹腔注射 

分 类 号：R332[医药卫生—人体生理学] R284.1[医药卫生—基础医学]