水稻草矮病毒侵染的水稻根系差异蛋白鉴定  

Identification of Differentially Expressed Proteins in Roots of Rice(Oryza sativa)Infected with Rice grassy stunt virus

作　　者：丁新伦[1] 谢荔岩[1] 张洁[1] 吴祖建[1] 

机构地区：[1]福建农林大学植物病毒研究所/福建省植物病毒重点实验室,福州350002

出　　处：《中国水稻科学》2016年第4期356-362,共7页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(31301640);国家科技支撑计划资助项目(2012BAD19B03)

摘　　要：为鉴定与水稻草矮病毒(Rice grassy stunt virus,RGSV)侵染引起的水稻根系发育不良的相关蛋白,解析根系发育不良症状的形成机理以及水稻草矮病毒与水稻互作的分子机制,采用双向荧光差异凝胶电泳(2D-DIGE)结合基质辅助激光解析串联飞行时间质谱技术(MALDI-TOF-MS)对RGSV侵染水稻后根系的差异表达蛋白进行分离和鉴定,并对鉴定的差异表达蛋白进行GO聚类分析和KEGG生物通路注释。结果表明,差异倍数大于2的蛋白质点有56个,其中,表达丰度升高的点有34个,表达丰度下降的点有22个;质谱成功鉴定出27个蛋白质点,分属于25种蛋白质。GO聚类分析表明差异表达蛋白涉及14个生物学过程,在生物功能上分属10类。细胞组件分析显示差异表达蛋白定位于不同的细胞部位。KEGG通路分析显示差异蛋白参与了12个生物通路。In order to find the proteins related to root maldevelopment caused by Rice grassy stunt virus（RGSV）,elucidate the mechanism of root maldevelopment and better understand the molecular basis of interaction between RGSV and host rice,2D-DIGE coupled with MALDI-TOF-MS was used to screen and identify differentially expressed proteins between RGSV-infected and RGSV-uninfected rice roots.The acquired proteins were further analyzed using GoMiner programs and KEGG pathway database.The results showed that 56 protein spots（differential ratio〉2）were differentially expressed,including 34up-regulated and 22down-regulated protein spots.Among them,27 differentially expressed protein spots which belonged to 25 proteins were identified,and these proteins were mainly involved in 14 biological processes,10 molecular functions and 12 KEGG pathways.

关 键 词：水稻草矮病毒 水稻根系 差异表达蛋白 双向荧光差异凝胶电泳 基质辅助激光解析串联飞行时间质谱技术 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]