GC-MS检测生物检材中的敌敌畏  

Method of GC-MS in Detecting the Dichlorvos in the Biological Samples

作　　者：解润芳[1] 李玉雯 解继明 杨凯润 王欣[1] 董继攀 

机构地区：[1]昆明医科大学法医学院,云南昆明650500 [2]云南省第三人民医院内分泌科,云南昆明650011 [3]云南省第三人民医院心胸外科,云南昆明650011

出　　处：《中国卫生产业》2016年第25期105-107,共3页China Health Industry

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项资金项目(2014FB013;2016FZ007)

摘　　要：目的建立一种简便、快速、准确、可靠的GC-MS检测生物检材中敌敌畏的方法。方法用无水Na2SO4把生物检材拌成干沙状以后,用氯仿-丙酮(6:1)进行提取分离,GC-MS对样品进行分离分析,用总离子流图(TIC图)中敌敌畏的保留时间及质谱图中对应的特征离子碎片定性。结果该方法简单易操作,背景干扰小,分离效果好,分析速度快,敌敌畏的保留时间为4.895 min、特征离子碎片(m/z)为79,109及185。结论该方法可用于法医毒物分析生物检材中敌敌畏的检测,结果令人满意。Objective To establish a simple, rapid, accurate and reliable method of detecting the dichlorvos in the biologi-cal samples by GC-MS. Methods The biological samples were given extraction and isolation by chloroform-acetone （6：1） af-ter they were mixed in the dry sand shape, the samples were given isolation and analysis by GC-MS, and were given quali-tative analysis by the dichlorvos retention time in the total ion current chromatogram （TIC picture） and corresponding char-acteristic ion fragments in the mass spectrum picture. Results The method is easy to operate with small background inter-ference, good isolation effect and rapid analysis speed, and the retention time of dichlorvos was 4.895min and the character-istic ion fragments （m/z） were 79, 109 and 185. Conclusion The result of the method in detecting the dichlorvos in the bio-logical samples in the forensic toxicological analysis is satisfactory.

关 键 词：法医毒物分析 GC-MS 生物检材 敌敌畏 

分 类 号：R446.1[医药卫生—诊断学]