TSLPR抗体抑制ApoE敲除小鼠动脉粥样硬化血栓形成  

TSLPR antibody inhibits platelet function and atherothrombosis in Apo E^(-/-) mice

作　　者：王博远[1] 吴纯[1] 何少林[1] 黄恺[1] 李大主[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心血管内科,武汉430022

出　　处：《中国免疫学杂志》2016年第11期1569-1572,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(81170258);湖北省自然科学基金重点项目资助

摘　　要：目的:探讨TSLPR抗体在抑制动脉粥样硬化血栓形成中的作用。方法:8周龄载脂蛋白E^(-/-)小鼠,给予高脂饲养12周,期间分别周期性尾静脉注射TSLPR抗体和同型无关IgG抗体,检测各组小鼠动脉粥样硬化血栓形成时间,血小板聚集和释放功能,血小板膜糖蛋白表达。结果:与对照组相比,TSLPR抗体处理组血栓形成时间明显长,血小板聚集功能和释放功能明显低,血小板活化指标CD62p、CD63、PAC-1表达明显低,静息指标CD42b表达明显高。结论:静脉注射TSLPR抗体可以明显抑制血小板聚集功能和释放功能,降低血小板活化程度,从而抑制动脉粥样硬化血栓形成。Objective： To explore the role of TSLPR antibody in the atherothrombosis inhibition. Methods： 8 weeks old Apo E＋（-/-） mice were fed with high-cholesterol diet for 12 weeks. During this time,mice in different groups were injected periodically with TSLPR antibody or isotype IgG respectively. The time of atherothrombosis,platelet aggregation function,platelet release function,and the expression of platelet membrane glycoprotein in different groups were detected. Results： Compared with those in the IgG control group,in the TSLPR antibody group the time of atherothrombosis was prolonged,the function of platelet aggregation and release was weakened,the expression of CD62 p,CD63,PAC-1 was decreased,the expression of CD42 b was increased. Conclusion： TSLPR antibody injection could inhibit atherosclerosis thrombus formation via weakening platelet aggregation and release function and repressing platelet activation.

关 键 词：TSLPR抗体 动脉粥样硬化 动脉粥样硬化血栓形成 血小板 

分 类 号：R392.11[医药卫生—免疫学]