猪圆环病毒2型减少猪肺泡巨噬细胞中伪狂犬病疫苗载量和CD80-CD86基因转录  

Porcine circovirus type 2 decreases the load of pseudorabies vaccine and gene transcription of CD80-CD86 in porcine alveolar macrophages

作　　者：杨倩[1] 周维依 于红欣[1] 周双海[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206 [2]北京巴基斯坦使馆学院,北京100600

出　　处：《北京农学院学报》2016年第4期52-55,共4页Journal of Beijing University of Agriculture

基　　金：北京市农委"菜篮子"能力提升工程项目(20150203-13)

摘　　要：【目的】探明猪圆环病毒2型(PCV2)干扰伪狂犬病疫苗(PRV)免疫效果的可能机制。【方法】用PCV2与PRV在体外分别单接种和共接种仔猪肺泡巨噬细胞(AM),在接种后不同时间用荧光定量PCR技术检测PRV载量与共刺激分子CD80-CD86mRNA水平的动态变化。【结果】PRV组上清中PRV载量在接种后都显著(P<0.05)高于PCV2+PRV组。与对照组相比,在接种后48h内,PRV组CD80mRNA水平在接种后增加并在48h时达到显著水平,而PCV2组与PCV2+PRV组的CD80 mRNA水平都显著减少;在接种后48h内,CD86mRNA水平在PRV组都显著增加,在PCV2+PRV组则都显著减少,但在PCV2组仅在接种后48h显著减少。【结论】PCV2能够减少猪AM中PRV载量与CD80-CD86基因转录,这可能是PCV2抑制PRV免疫效果的机制之一。【Objective】The aim of this study is to investigate the possible mechanism of the porcine circovirus type 2（PCV2）-derived interference with the efficacy of pseudorabies vaccine（PRV）.【Methods】Porcine alveolar macrophage（AM）was inoculated with PCV2 and PRV alone or combined in vitro,the changes in the load of PRV and the mRNA level of co-stimulating molecules CD80-CD86 at different hours post inoculation（HPI）was detected by fluorescent quantitative PCR.【Results】The load of PRV in the supernatant of the PRV group was significantly（P〈0.05）higher than that of the PCV2＋PRV group after inoculation.Within 48 hours after inoculation,as compared to the control group,the mRNA level of CD80 increased and reached to a significant level at 48 HPI in the PRV group,but all decreased significantly in both the PCV2 group and the PCV2＋PRV group;meanwhile,the CD86 mRNA levels increased significantly in the PRV group and reduced significantly in the PCV2＋PRV group,while decreased significantly only at 48 HPI in the PCV2 group.【Conclusion】The PCV2 reduced the load of PRV and the gene transcription of CD80-CD86 in porcine AM,which may be a possible mechanism of the PCV2-derived interference with the efficacy of PRV.

关 键 词：猪圆环病毒2型 伪狂犬病疫苗 肺泡巨噬细胞 CD80-CD86 

分 类 号：S852.695.2[农业科学—基础兽医学]