观赏海棠不同品种MYB10启动子结构分析  

The study on promoter structure of MYB10 in different crabapple cultivars

作　　者：庞慧[1] 田佶[1] 姚允聪[1] 

机构地区：[1]北京农学院植物科学技术学院/北京市果树良种繁育中心/北京林果业生态环境功能提升协同创新中心,北京102206

出　　处：《北京农学院学报》2016年第4期76-80,共5页Journal of Beijing University of Agriculture

基　　金：科技创新服务能力建设-协同创新中心-林果业生态环境功能提升协同创新中心(2011协同创新中心)(市级)(PXM2016_014207_000038)

摘　　要：【目的】观赏海棠叶片色泽类型多样,为了研究McMYB10基因对不同叶色观赏海棠品种呈色的影响。【方法】利用PCR克隆的方法,从两个极端叶色观赏海棠品种中克隆得到两个McMYB10启动子,同时对这两个启动子在5个不同叶色类型的观赏海棠分布进行检测。【结果】McMYB10启动子在不同观赏海棠品种中存在两种不同的类型,即R1型和R6型;在McMYB10启动子中存在逆境、激素、光等多种顺式作用元件,同时在变色类观赏海棠品种中,R1和R6型启动子都存在。【结论】R1和R6型启动子在不同叶色的观赏海棠品种中,可能对叶片的呈色起较为重要的作用。【Objective】Ornamental crabapples have diverse colors of leaves.To explore the molecular mechanism of McMYB10 affecting the coloration of leaves in different crabapple cultivars.【Methods】We cloned two McMYB10 promoters,R6 and R1,from the ever-red leaved Malus crabapple cultivar‘Royalty＇and ever-green leaved Malus crabapple cultivar‘Flame＇by using the method of PCR cloning,and also tested the distribution of the promoter structure in 5crabapple cultivars.【Results】The results showed that R1 and R6are exist in different crabapple cultivars,and the promoter of McMYB10 has many cis-elements that maybe induced by stress,hormone and light.Meanwhile,the spring-red crabapple cultivar has both R1 and R6promoters.【Conclusion】R1and R6 promoter play an important role in the leave coloration in crabapple.

关 键 词：观赏海棠 McMYB10 启动子 

分 类 号：S661.4[农业科学—果树学]