甘肃、宁夏奶牛新孢子虫血清流行病学调查及风险因素分析  

SEROPREVALENCE AND RISK FACTORS OF NEOSPORA CANINUM INFECTION IN DAIRY CATTLE IN GANSU AND NINGXIA

作　　者：谭启东 魏邦香 陈兵 张晓轩[1] 杨言川[1] 秦思源[1] 朱兴全[1] 徐前明[3] 周东辉[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,兰州730046 [2]北京出入境检验检疫局动物隔离场,北京101312 [3]安徽农业大学动物科技学院,合肥230036 [4]榆中县动物疾病预防与控制中心,榆中730000

出　　处：《中国动物传染病学报》2016年第5期46-50,共5页Chinese Journal of Animal Infectious Diseases

基　　金：国家科技支撑计划项目(2012BAD12B07);甘肃省创新研究群体计划项目(1210RJIA006)

摘　　要：本研究旨在查明甘肃省与宁夏回族自治区奶牛新孢子虫的感染情况并分析影响其感染的风险因素。采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)方法检测了甘肃省榆中县、宁夏青铜峡市和宁夏吴忠市3个地方总计1657份奶牛血清样品,并应用流行病学调查及统计学方法对影响奶牛新孢子虫感染的因素进行了分析。流行病学调查结果显示,奶牛新孢子虫抗体总阳性率为8.93%;应用Logistic回归分析评估奶牛新孢子虫感染的风险因素,结果显示奶牛所处地区是影响新孢子虫感染的风险因素(P<0.05)。调查分析表明,甘肃和宁夏地区奶牛新孢子虫普遍流行。应当提高对调查地区奶牛新孢子虫感染的重视,采取适当的综合控制方法和有效的管理措施以防控奶牛新孢子虫病,以保证奶牛养殖业的经济效益。The objective of the present investigation was to examine seroprevalence of N. caninum in dairy cattle in Gansu province and Ningxia Hui Autonomous Region（NXHAR）, Northwest China for assessment of the associated risk factors. In this study, a total of 1657 serum samples were collected and tested for the antibodies of N. caninum using a commercially available ELISA kit. The seropositive percentage was 8.93%（148/1657）. Then, multiple logistic regression was used to analyze the risk factors associated with seroprevalence.The results of the present survey indicated the widespread of N. caninum infection in Gansu and NXHAR. Thus, comprehensive control strategies and efficient management measures for N. caninum infection should be implemented to increase profit of the dairy cattle industry.

关 键 词：新孢子虫 ELISA 流行病学 奶牛 甘肃 宁夏 

分 类 号：S852.723[农业科学—基础兽医学]