禾本科牧草及草坪草原生质体培养与融合技术研究进展  

Research Progress in Protoplast Culture and Fusion Technologies of Gramineous Forage and Turfgrass Plants

作　　者：牛奎举 马晖玲[1] 

机构地区：[1]甘肃农业大学草业学院草业生态系统教育部重点实验室中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草地学报》2016年第4期738-746,共9页Acta Agrestia Sinica

基　　金：国家自然科学基金(草地早熟禾种间体细胞杂交的研究-31160482)资助

摘　　要：禾本科牧草与草坪草在可持续农业发展、城市绿化、生态保护等方面起着至关重要的作用。植物体细胞杂交是有性杂交的重要补充,在转移由多基因控制和尚未克隆目标基因控制的性状方面具有极大的潜力。本文综述了禾本科牧草及草坪草的原生质体培养与融合技术,包括原生质体分离材料的选择、培养基成分、培养密度及培养方式、融合的方法与方式。同时,对禾本科牧草及草坪草体原生质体培养与融合技术的应用前景作出展望。Gramineous forage and turf grass plants play important roles in supporting the sustainable agriculture developments,afforestation of city and environmental protection.Somatic hybridization,which has great potential in transferring the target characters controlled by multigenes or uncloned genes,is an important supplement to the sexual hybridization.In this paper,the technologies of protoplast culture and fusion of gramineous forage and turf grass were reviewed.It included selecting the materials for protoplast isolation,culture media,culture density and methods,fusion methods.Moreover,the prospects of the protoplast culture and fusion technologies of gramineous forage and turf grass plants were made.

关 键 词：禾本科牧草 草坪草 原生质体培养 原生质体融合 

分 类 号：Q813.2[生物学—生物工程] S813.3[农业科学—畜牧学]