谷子硒结合蛋白基因及其与抗旱性的关系  

Selenium-binding protein gene family and its relationship with drought tolerance in foxtail millet

作　　者：武彩娟 苏彦冰 程璐[1] 王钇杰 元旭朝 高炜[1] 李红英[1,2,3] 

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]农业部黄土高原作物基因资源与种质创制重点实验室,山西太原030031 [3]杂粮种质资源发掘与遗传改良山西省重点实验室,山西太谷030801

出　　处：《山西农业大学学报（自然科学版）》2016年第12期850-854,共5页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金(31471556);山西农业大学科技创新基金(2014022.2014YZ2-5)

摘　　要：[目的]土壤与肥料中的硒元素进入植物体后,与植物中的蛋白质和活性有机成分结合成为植物有机硒,即植物硒结合蛋白。本研究以硒结合蛋白结构和功能为基础,探索其与谷子抗旱的关系。[方法]以谷子耐旱品种勾勾母鸡咀(GG)和干旱敏感品种晋汾16(JF16)为试材,以转录组中硒结合蛋白基因家族中10个基因为对象,利用生物信息学分析其基因组基本信息、基因间亲缘关系及启动子顺式元件,并对Seita.4G100300基因进行表达水平分析。[结果]发现谷子硒结合蛋白基因家族中基因启动子上游1 500bp的调控元件具有响应胁迫的多种功能(ABA、Ethylene、GA、MeJA、Light、MYB、SA、热和低温等);经过干旱处理,Seita.4G100300基因在GG和JF16中表达水平均增加,且其在耐干旱品种GG中表达水平的增幅显著大于干旱敏感品种JF16。[结论]谷子硒结合蛋白基因家族在氨基酸序列和表达模式方面具有较为丰富的多样性。本研究有助于我们理解硒结合蛋白的结构和功能,为进一步研究硒结合蛋白与植物抗逆性关系提供理论依据。[Objective]Selenium in soil and fertilizer becomes selenium-binding protein when it is absorbed by plants and then combined with proteins and active organic ingredients.Based on the structure and function of selenium-binding protein,the study explored the relationship between it and drought tolerance.[Methods]In the study,drought tolerant variety（GG）and drought sensitive variety（JF16）were used as test materials,and 10 genes of selenium-binding protein gene family were studied.Using bioinformatics,the basic information of the genes,the genetic relationship among the genes and the cis-acting elements in promoters were analyzed.The expression pattern of the Seita.4G100300 gene was analyzed.[Results]It was found that the regulatory elements within the upstream 1500 bp in promoter of seleniumbinding protein gene family in foxtail millet had the functions of responding to multiple stresses（ABA,Ethy,GA,MeJA,Light,MYB,SA,heat,low temperature）.After drought tolerance,the expression levels of Seita.4G100300 gene increased in both GG and JF16.And the rise of expression level in GG（drought tolerant variety）was significantly higher than that in JF16（drought sensitive variety）.[Conclusion]The selenium-binding protein gene family has rich diversity in the amino acid sequence and expression pattern.The study will help us to understand the structure and function of selenium-binding protein.And it will provide a theoretical basis for the further study of the relationship between selenium-binding protein and plant stress resistance.

关 键 词：谷子 硒结合蛋白 抗旱性 

分 类 号：S515[农业科学—作物学] Q94[生物学—植物学]