淫羊藿水提取物联合抗菌药对耐药大肠杆菌的体外抑制效果  

In vitro inhibitory effect of Epimedium brevicornu Maxim.water extract combined with antimicrobials on drug-resistant Escherichia coli

作　　者：胡梅[1] 梁政[1] 黎天良 秦风伟 罗远燕 吴永继 刘增援 赵雨川 司红彬[1] 

机构地区：[1]广西大学动物科学技术学院,南宁530005

出　　处：《南方农业学报》2016年第10期1778-1783,共6页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(31460675);广西自然科学基金项目(2013GXNSFAA019070);南宁市科技攻关项目(20141295);广西大学大学生创新创业训练计划项目(201610593258)

摘　　要：【目的】探究淫羊藿水提取物联合抗菌药对耐药大肠杆菌的体外抑菌效果,为兽医临床中西联合用药提供参考依据,也为中药在抑制耐药菌株上的开发研究打下基础。【方法】采用二倍微量肉汤稀释法测定淫羊藿水提取物及各抗菌药对产超广谱β-内酰胺酶(ESBLs)大肠杆菌的最小抑菌浓度(MIC),然后以微量肉汤棋盘稀释法测定淫羊藿水提取物与抗菌药联用后的部分抑菌浓度指数(FICI)。【结果】淫羊藿水提取物与粘杆菌素、头孢曲松、头孢他啶、头孢噻呋、阿莫西林、磷霉素、阿米卡星、氟苯尼考、乙酰甲喹、林可霉素联用后能有效降低药物的MIC,对应的MIC均降低4倍以上,联合用药的FICI分别为0.13、0.26、0.26、0.25、0.51、0.51、0.50、0.75、0.75和0.75,对产ESBLs大肠杆菌的抑制效果表现出协同或相加作用;以其联合诱导产ESBLs大肠杆菌传代,从第1代即能明显降低这些抗菌药的MIC,且稳定至第3代。【结论】淫羊藿水提取物与粘杆菌素、头孢他啶、头孢曲松、头孢噻呋、阿莫西林、磷霉素、阿米卡星、氟苯尼考、乙酰甲喹、林可霉素联用是通过协同或相加作用而提高抗菌药对产ESBLs大肠杆菌的抑制效果,但不宜与磺胺类、喹诺酮类联合用药。【Objective】The in vitro antibacterial effect of water extract from Epimedium brevicomu Maxim,combined with antimicrobials on drug-resistant Escherichia coli was studied,in order to provide reference basis for combination of traditional Chinese and western medicines in veterinary clinic,also lay foundation for development and research of traditional Chinese medicine against drug-resistant strains.【 Method 】The microbroth dilution assay methods were used to determine minimal inhibitory concentration（MIC） of water extract from E.brevicomu and antimicrobials against extended spectrum beta-lactamases（ESBLs）-producing E.coli.Then their fractional inhibitory concentration index（FICI） was determined with microdilution checkerboard techniques.【 Result 】 After combining antimicrobials with water extract of E.brevicomu,the MICs of colistin,ceftriaxone sodium,ceftazidime,ceftiofur,amoxicillin,fosfomycin,amikacin,florfenicol,maquindox,clindamycin were decreased more than four times,the FICIs were 0.13,0.26,0.26,0.25,0.51,0.51,0.50,0.75,0.75 and 0.75,so water extract from E.brevicomu combined with antimicrobials showed synergistic or additive inhibitory effect on ESBLs-producing E.coli.Furthermore,after water extract from E.brevicomu combined with antimicrobials were used to induce passage of ESBLs-producing E.coli,it was found that medicine combination could significandy reduce the MICs of antibiotics from the 1^（st） generation to the 3^（rd） generation.【Conclusion】The combination of water extract from E.brevicomu with colistin,ceftriaxone sodium,ceftazidime,ceftiofur,amoxicillin,fosfomycin,amikacin,florfenicol,maquindox,clindamycin can improve inhibitory effect on ESBLs-producing E.coh by their synergistic or additive effect,but is inadvisable to combine with sulfa and quinolone.

关 键 词：淫羊藿 抗菌药 产ESBLs大肠杆菌 联合用药 抑制效果 

分 类 号：S853.75[农业科学—临床兽医学]