夹竹桃内生真菌R22的次生代谢产物研究  

Studies of secondary metabolites from an endophytic fungi R22 of Nerium indicum

作　　者：马养民[1] 乔珂[1] 李梦云[1] 郭林新[1] 范超[1] 

机构地区：[1]陕西科技大学教育部轻化工助剂化学与技术重点实验室,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2016年第6期125-129,134,共6页Journal of Shaanxi University of Science & Technology

基　　金：陕西省科技厅自然科学基础研究计划项目(2014JZ003)

摘　　要：为探究夹竹桃内生真菌R22次生代谢产物的结构和活性,采用硅胶柱色谱、Sephadex LH-20柱色谱、重结晶等方法对该菌株的发酵产物进行分离、纯化,并结合所得化合物的理化性质和波谱数据鉴定其结构.通过二倍稀释法对其次生代谢产物进行抑菌活性测试.最终从其发酵产物中分离得到六个化合物.经鉴定分别为丁二酸(1)、十六烷酸α-单甘油酯(2)、3-甲基-4-喹唑啉酮(3),cyclopenol(4)、尿囊素(5)、腺苷(6).其中化合物3为首次从自然界中分离得到,化合物5对植物病原真菌具有一定的抑菌活性.To explore the structure and activity of secondary metabolites of endophytic fungus from R22 strain in Nerium indicum,the secondary metabolities from R22 were isolated by silica gel column,Sephadex LH-20 column chromatography,recrystallization,and so on.Their structures were determined by their physicochemical properties and spectrum data.And an MIC method was used in antibacterial activity test.Six compounds from fermentation of R22 were identified as succinicacid（1）,daturic acid-2,3-dihydroxypropylester（2）,3-methylquinazolin-4（3H）-one（3）,cyclopenol（4）,allantoin（5）,adenosine（6）.Compound 3was isolated from the natural world for the first time.Compound 5revealed some antimicrobial activity against the phytopathogeic fungi.

关 键 词：夹竹桃 内生真菌 青霉属 次生代谢产物 抑菌活性 

分 类 号：O629[理学—有机化学]