压疮模型大鼠血清和组织中超氧化物歧化酶与一氧化氮的实验观察  

Experimental observation of SOD and NO in serum or tissue in rat model with pressure ulcer

作　　者：徐云侠[1] 李萍[1] 储明子[1] 钟政荣[2] 

机构地区：[1]上海交通大学医学院附属第九人民医院肾脏内科,上海201999 [2]上海交通大学医学院附属第九人民医院检验科

出　　处：《中华全科医学》2016年第12期1998-2001,共4页Chinese Journal of General Practice

基　　金：安徽省教育厅自然科学研究重大项目(KJ2015-ZD31);安徽省自然科学基金项目(1308085-MH124);蚌埠医学院自然科学研究项目(byky1231)

摘　　要：目的前期实验结果显示,压迫超过3 h时,试验大鼠的受压组织明显受损。进一步实验观察不同压迫时间下,SD大鼠血清和组织中的超氧化物歧化酶(SOD)和一氧化氮(NO)结果差异,为寻找压疮患者最佳的翻身时间提供实验室依据。方法将48只SD大鼠分为对照组和实验组,每组8只,对照组未作任何处理,实验组又分为压迫1小时组(即压迫1 h后再解压1 h,再压迫、解压各1 h,依此循环,直至24 h实验结束;简称1 h组,后同)、2小时组(2 h组)、3小时组(3 h组)、4小时组(4 h组)、6小时组(6 h组),共5组,实验组各组的压迫时间与解压时间相等(即缺血和再灌注时间相等),各组缺血和再灌注的总试验时程均为24 h,实验结束后收集各组大鼠的血清和组织,用酶联免疫吸附试验(ELISA)测定血清和组织匀浆液中的SOD和NO的浓度,比较各组SOD和NO的结果差异。结果与对照组相比,3 h组、4 h组和6 h组血清和组织中的SOD均明显降低,而NO明显升高,差异均具有统计学意义(P<0.05),而且随着压迫时间的延长,差异越明显。但1 h组和2 h组的结果差异无统计学意义(P>0.05)。结论压迫超过3 h,大鼠血清和组织中的SOD和NO均发生明显改变,因此,SOD和NO可以作为预测压疮发生的早期无创实验室风险指标。Objective Our previous results showed that the pathological damage of the skin and tissue exposure to pressure could he caused by 3 h-compression in rats. Further observation was needed for the level of superoxide dismutase （SOD） and nitric oxide（NO） in serum or tissues in SD rats with pressure ulcer so as to screen the optimal frequency of repositioning and risk assessment markers for pressure ulcer. Methods Total 48 SD rats were divided into six groups （ 8 in each group） ： the control（untreated） , and pressure for one hour（ 1 h） , two hours （ 2 h）, three hours （ 3 h）, four hours （4 h） or six hours（6 h） followed by the same time interval. After 24h,blood and local tissues samples from compression area were prepared for SOD and NO determination by enzyme-linked immunosorbent assay （ELISA）. And statistical significance analysis of variance for SOD and NO were tested by Student＇ s t test. Results Compared with the control,lower activity of SOD and higher level of NO were found in 3 h ,4 h and 6 h groups. And the differences were statistically significant （P 〈 0.05 ） and time dependence. However, there were not statistical significanees between the control and the 1 h or 2 h group（ P 〉 0.05 ）. Conclusion A pressure duration for 3h is a threshold for the levels of SOD and NO above which pressure damage may occur. Therefore, SOD and NO may be regarded as the early-stage and noninvasive markers in risk assessment at pressure ulcer.

关 键 词：压疮 超氧化物歧化酶 一氧化氮 

分 类 号：R3[医药卫生—基础医学] R446.61