河南省HBV基因型、基因亚型及血清型分布调查  

Distribution of HBV genotypes,subgenotypes,and serotypes among the HBV-infected population in Henan

作　　者：郭永豪[1] 杨建辉[1] 张璐[1] 董蒲梅[1] 徐瑾[1] 叶莹[1] 张延炀[1] 王哲[1] 

机构地区：[1]河南省疾病预防控制中心,河南郑州450016

出　　处：《中国病原生物学杂志》2016年第11期1018-1021,共4页Journal of Pathogen Biology

基　　金：河南省医学科技攻关计划项目(No.201203157);河南省基础与前沿技术研究计划项目(No.122300410054)

摘　　要：目的了解河南省人感染HBV的基因型、基因亚型及血清型的分布。方法采用巢式PCR法对44例HBV感染者血样进行S基因扩增并测序,应用Neighbor-Joining构建系统进化树,分析其基因型和基因亚型;应用Magnius和Norder法进行血清型分析。结果 44例HBV感染者中检出C基因型36例(81.8%),B型8例(18.2%)。B基因型全部为B2亚型,adw2血清型。C基因型中有33例(占75.0%)为C2亚型,adrq+血清型占97.2%(35/36),adrq-血清型占2.8%(1/36)。结论河南省人感染HBV以C基因型为主,其次是B基因型;C基因型中以C2亚型和adrq+血清型为主,B基因型中以B2亚型和adw2血清型为主。Objective To investigate the distribution of hepatitis B virus（HBV）genotypes,subgenotypes,and serotypes among the HBV-infected population in Henan Province. Methods Forty-four S genes of HBV were amplified with nested PCR and then directly sequenced.A phylogenetic tree was constructed using the neighbor-joining method and analyzed,and then the viral genotypes and subgenotypes were identified.Serotypes were determined using the approach of Magnius and Norder. Results In 44 patients infected with HBV,36（81.8%）were infected with the C genotype and the remaining 8（18.2%）were infected with the B genotype.Genotype C isolates were mainly subgenotype C2 and serotype adrq＋.Genotype B isolates were subgenotype B2 and serotype adw2. Conclusion The major genotypes of HBV prevalent among HBV-infected people in Henan Province were C and C.Genotype C isolates were mainly subgenotype C2 and serotype adrq＋ while genotype B isolates were mainly subgenotype B2 and serotype adw2.

关 键 词：乙型肝炎病毒 基因型 基因亚型 血清型 

分 类 号：R373.21[医药卫生—病原生物学]