硼酸抗白念珠菌作用的试验研究  

Experimental study on the effect of boric acid against Candida albicans

作　　者：高吉[1] 潘丽娜[1] 郭东东[1] 魏羽佳[1] 

机构地区：[1]贵州医科大学附属医院皮肤科,贵阳550004

出　　处：《中国真菌学杂志》2016年第5期275-278,288,共5页Chinese Journal of Mycology

基　　金：贵州省优秀科技教育人才省长专项资金(【2011】28号)

摘　　要：目的探讨硼酸对白念珠菌临床分离株菌株的生长状态和细胞形态的影响。方法用加入0%、0.1%、0.15%硼酸(w/v)的Lee’s glucose培养基于25℃和37℃分别培养8株临床分离的白念珠菌,观察各菌株在不同培养条件下生长状况。结果 25℃培养条件下,0.1%的硼酸明显抑制白念珠菌生长,其中对HJ065的抑制效果最明显,0.15%的硼酸进一步抑制白念珠菌生长,并且SC5314、HJ058菌株的菌丝生长能力随硼酸浓度增高而减弱。37℃培养条件下,硼酸对白念珠菌生长状态和菌丝生长能力的抑制效果较25℃更明显。相同培养条件下,硼酸对不同CAI重复数的白念珠菌的抑制效应存在差异。结论硼酸对白念珠菌临床分离菌株的抑制作用表现出明显菌株的差异性。其抑制作用与培养温度有关:表现为生理温度下抑制作用更明显。硼酸对8株白念珠菌抑制作用与CAI的重复数无明显相关性。Objective We aimed to study the different effects of boric acid on cell growth and cell morphology in eight clinical isolates of Candida albicans.Method Lee＇s glucose media containing different concentration of boric acid（0%,0.1%,0.15%）were used for cell growth of Candida albicans.The growth of the strains in different culture conditions were observed.Result When treated with 0.1% of boric acid at 25℃,the growth of eight strains were extremely inhibited,especially HJ065.When treated with 0.15% of boric acid,the growth of all strains were further inhibited.Boric acid with high concentration impacted the filamentation growth of HJ058 and SC5314.At 37℃,the inhibition effect of boric acid on the growth of C.albicnas was more obvious than at 25℃.Under the same culture condition,the effect of boric acid had no relationship with the number of CAI in C.albicans.Conclusion The inhibitory effect of boric acid on the clinical isolates of Candida albicans showed significant differences among the strains,and also was related to the culture temperature.Under physiological temperature,C.albicans was more sensitivity to boric acid.There was no significant correlation between the inhibitory effect of boric acid and the CAI genotype of the 8strains of C.albicans we used.

关 键 词：白念珠菌 硼酸 耐受性 CAI 

分 类 号：R379.4[医药卫生—病原生物学]