黑丝羽乌骨鸡DMA细管冻精冷冻方法的研究  

Study on the freezing method of straw frozen semen from black silkies with N-N-dimethylacetamide(DMA)

作　　者：蔡文杰[1] 李剑波 张翠永 邱美珍[1] 邓溯顺[1] 燕海峰[1,3] 

机构地区：[1]湖南省畜牧兽医研究所,长沙410131 [2]湖南光大牧业科技有限公司,长沙410131 [3]湖南天心黄鸡育种有限公司,长沙410143

出　　处：《黑龙江畜牧兽医（下半月）》2016年第10期196-199,共4页

基　　金：国家国际科技合作专项(2013DFG31810);湖南省科技计划(2014WK2008);长沙市科技计划(K1307134-24)

摘　　要：为建立黑丝羽乌骨鸡精液合适的冷冻保存程序,选择16只35周龄黑丝羽乌骨鸡公鸡,采集单只公鸡精液,从精液稀释基础液BPSE(Beltsville Poultry Semen Extender)、Lake’s与FEB、加入冷冻保护剂N,N-二甲基乙酰胺(DMA)后平衡时间和细管离液氮面的距离等方面研究冻精解冻后精子活力。结果表明:1)3种稀释液的效果依次为BPSE、Lake’s与FEB,前两者差异不显著,但BPSE液显著高于FEB液(P<0.05);2)平衡1 min和10 min组的活力显著高于平衡20 min、30 min组(P<0.05);3)离液氮面1 cm和2 cm组的活力显著高于离液氮面3 cm和4 cm组(P<0.05)。To establish a suitable cryopreservation program for semen from black silkies, 16 black silkies roosters at 35 weeks of age were select- ed, and the semen was collected from a single rooster. The semen was diluted using Beltsville poultry semen extender （BPSE） , Lake＇ s and FEB, and then was added with N -N - dimethylacetamide （DMA）, respectively. The sperm motility after thawing was studied from the as- pects of equilibration time, the distance of straw to liquid nitrogen surface. The results showed that the effects of three diluents in descending or- der were as follows： BlaSE, Lake＇ s and FEB, and there was no significant difference between BPSE and Lake＇ s, but the effect of BPSE was signifieantly higher （P 〈 0.05 ） than that of FEB. The sperm motilities in the groups with 1 -min and 10 -min equilibration time were signifi- cantly higher （ P 〈 0.05 ） than those of the groups with 20 - min and 30 - min equilibration time. The sperm motilities in the groups with the 1 cm and 2 cm distances of straw to liquid nitrogen surface were significantly higher （ P 〈 0.05 ） than those in the groups with 3 cm and 4 cm dis- tances of straw to liquid nitrogen surface.

关 键 词：黑丝羽乌骨鸡 精液 N N-二甲基乙酰胺(DMA) 细管 冻精 

分 类 号：S831.3[农业科学—畜牧学]