一种烟草细菌性病害病原菌分离培养及致病性测定  

Isolation,Culture and Pathogenicity Identification of Pathogen of A Tobacco Bacterial Disease

作　　者：蒋承耿 瞿鸿飞 王忠宇 杨应波 李长权 杨再福[2] 龙友华[2] 

机构地区：[1]贵州省烟草公司六盘水市公司,贵州六盘水553000 [2]贵州大学农学院,贵州贵阳550025

出　　处：《山东农业科学》2016年第11期72-75,共4页Shandong Agricultural Sciences

基　　金：贵州省烟草公司科技项目"六盘水烟区一种未知烟草病害鉴定与识别"(201108)

摘　　要：从贵州六盘水烟区采集感病烟株,采用组织分离法进行病原菌分离纯化,并将培养条件进行优化,以南江3号烟草品种为试材,进行病原菌致病性测定。结果表明,该菌培养最适Na Cl浓度4%,葡萄糖浓度10%,p H值5~7,培养温度25~30℃,最佳侵染温度20~25℃,相对湿度70%以上,该病害应属于中温高湿侵染病害。The isolation and purification of pathogen of a tobacco bacterial disease was conducted from tobacco- growing areas in Liupanshui of Guizhou Province using the issue separation method. Its culture conditions were optimized,and the pathogenicity was measured with Jiangnan 3 as material. The results showed that the optimum growth conditions of this pathogen were 4% of salt concentration,10% of sugar concentration,5 to 7 of p H value,and 25 ~ 30℃ of cultivation temperature. And the best infesting conditions of this pathogen were 20 ~ 25℃ of infesting temperature,and more than 70% of relative humidity. Thus,the disease was easy to infection under middle temperature and high humidity conditions.

关 键 词：烟草 病原菌 分离培养 致病性 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]