己烷雌酚抗原合成和多克隆抗体制备  

Synthesis of Antigen and Preparation of Multi-colonial Antibody Against Hexoestrol

作　　者：李宁 张巍 张治成 

机构地区：[1]哈尔滨市兽药饲料监察所,黑龙江哈尔滨150070

出　　处：《黑龙江农业科学》2016年第10期83-86,共4页Heilongjiang Agricultural Sciences

基　　金：哈尔滨市科技攻关计划资助项目(2014AB3BN041)

摘　　要：为了对己烷雌酚进行检测,采用混合酸酐法,合成具有己烷雌酚基本分子结构特性的半抗原。以己烷雌酚-牛血清白蛋白为抗原免疫家兔,获得了特异性的己烷雌酚多克隆抗体。结果表明:己烷雌酚半抗原与牛血清白蛋白偶联的摩尔比为12.93∶1。今后可将所得抗体用于间接竞争性酶联免疫吸附分析法用于己烷雌酚的检测。In order to detect hexoestrol,the antibody of HES was prepared with anhydride method for detecting HES residues later. Hapten with carboxylic that preserved the molecule structure character of diethylstilbestrol was synthesized. Polyclonal antibodies for diethylstilbestrol were obtained by immunizing rabbits with immune antigen that obtained by conjugated hapten with bovine serumalbum in DES-BSA. The results showed that hexoestrol hapten and bovine serum albumin （BSA）conjugated molar ratio was 12.93：1. In the future, the resul- ting antibodies could be used for the detection of indirect competitive enzyme-linked immunosorbent assays for the hexoestrol.

关 键 词：己烷雌酚 多克隆抗体 酶联免疫吸附分析 

分 类 号：S859.84[农业科学—临床兽医学]