湖南地方柚资源及其近缘种多样性的SRAP分子评价  

Molecular level's evaluation on the genetic diversity of pummelo germplasm and their relative species in hunan province based on srap markers

作　　者：李先信[1,2] 杨迎花 邹学校[4] 邓子牛[5] 

机构地区：[1]中南大学研究生院隆平分院,湖南长沙410125 [2]湖南省农业科学院园艺研究所,湖南长沙410128 [3]株洲市农业局,湖南株洲412007 [4]湖南省农业科学院蔬菜研究所,湖南长沙410125 [5]湖南农业大学园艺园林学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2016年第6期608-614,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省农业科学院科技创新团队项目(2014td04);中南大学博士创新基金项目(2340–77226)

摘　　要：以湖南本地柚类资源为试材,应用SRAP标记对41份柚类资源及5份近缘种的遗传多样性进行分析与鉴定。结果表明：平均每个引物组合可扩增出16.6条谱带,17对SRAP引物共扩增出283条谱带,其中多态性谱带235条,多态率为83.0%,基因多样度变幅为0.431 7-0.770 0,平均基因多样度为0.544 3;获得了21个基因型的37个SRAP特异性标记,不同引物组合可将42个基因型完全分开;柚类及近缘种等位基因平均数、平均杂合位点占比、SRAP表型杂合度（H0）分别为9.02、67.77%和0.343。聚类分析结果显示,41份柚种质及5份近缘种材料在遗传相似系数0.792处可分为6个组群：第1、2组群分别由24个和11个柚的地方品种构成;第3组群为柚的种间杂种类型,包括菠萝香柚、慈利金香柚、慈利甜柚2号和慈利水柚子;第4组群由慈利柚09–1、金瓜两杂种柚和酸橙、臭皮柑两近缘种组成;第5组群包括无核大红甜橙和温州蜜柑;第6组群为柠檬。综合SRAP标记结果与形态特征分析,认为慈利金香柚可能源于以柚类为母本、以橙类（或宽皮橘类）为父本的自然种间杂交后代。Genetic diversity of 41 pummelo（Citrus grandis Osbeck） germplasms and 5 relative species collected from Hunan province were identified and evaluated using the markers from sequence related amplified polymorphism（SRAP）. The results showed that the average number of 16.6 alleles could be amplified out from every primer combination, and total of 283 alleles were detected from 17 SRAP markers, 235 out of which presented polymorphic, accounted for 83.0%. Gene diversity value changed from 0.431 7 to 0.770 0 with an average of 0.544 3 for each primer, which showed a high level of genetic diversity among the analyzed germplasms. 37 specific SRAP polymorphic bands were scored, and 42 genotypes from the 46 germplasms were completely identified by SRAP markers. The mean number of alleles per locus（A）, percentage of polymorphic loci（P） and direct count heterozygosity（H0） of the genotypes from the pummelo germplasms and their relative species were 9.02, 67.77% and 0.343, respectively. 41 pummelo germplasms and 5 relatives could be clustered into 6 groups with the similarity coefficient of 0.792 by the method of UPGMA. Group 1 and group 2 were respectively composed of 24 and 11 pummelo varieties, group 3 was hybrid pummelos, group 4 was two of hybrid pummelos and two relatives, group 5 included two relatives of seedless red peel orange and Satsuma mandarin, and group 6 was only limon（Citrus limon（L.） Burm f.）. Parental origin of the hybrid pummelos were discussed in the paper as well.

关 键 词：柚 遗传多样性 种质鉴定 湖南 

分 类 号：S666.3[农业科学—果树学]