葛根芩连汤对抗生素相关腹泻肠道乳酸杆菌属的影响研究  

Influence of Gegenqinlian Decoction on Intestinal Lactobacillusin Antibiotic Associated Diarrhea Model Treatment

作　　者：刘茜明 杨光勇[1] 何光志[1] 田维毅[1] 蔡琨[1] 王平[1] 黄高[1] 梁建东[1] 王文佳[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《家畜生态学报》2016年第11期74-78,共5页Journal of Domestic Animal Ecology

基　　金：教育部科学技术研究重点项目(212153);贵州省教育厅创新群体重大研究项目(黔教合KY字2016036);贵州省高等学校工程研究中心项目(黔教合KY字20153377);贵阳中医学院2011协同创新中心资助(201403)

摘　　要：为了探讨葛根芩连汤对抗生素相关腹泻模型肠道中乳酸杆菌属的调理作用,运用氨苄青霉素灌胃建立巴马猪抗生素性腹泻动物模型,用葛根芩连汤进行治疗,处死巴马猪并收集不同位置的肠道内容物(包括十二指肠、空肠、回肠、盲肠和结肠)作为检测样品;通过建立定量检测乳酸杆菌属的荧光定量PCR方法,对葛根芩连汤治疗前后肠道内乳酸杆菌的动态变化进行检测。结果显示,与正常对照组相比,模型组肠道内乳酸杆菌属含量显著降低(P<0.01);与模型组相比,葛根芩连汤治疗组乳酸杆菌在各个肠段中数量明显上升(P<0.05)。长期使用抗生素会引起肠道内优势杆菌乳酸杆菌属数量明显减少;葛根芩连汤对抗生素相关腹泻模型肠道乳酸杆菌属具有调理作用。To investigate the effects of Gegenqinlian Decoction（GQD） on intestinal Lactobacillus in antibiotic associated diarrhea（ADD） animal model, the AAD model was established by Ampicillin. Experimental animals were randomly divided into control group, model group and treatment group; the treatment group was treated with GQD. After seven days and fourteen days, the content was collected from duode- num, jejunum, ileum, appendix and colon of Bama pigs. The Fluorescence Quantitative PCR（q-PCR）was used to detect the dynamics change of Lactobacillus in intestinal tract. Compared with the normal control group, Lactobacillus in ADD animal model group was significantly lower than that in control group （P〈 0.01）. The number of lactobacilli in various intestinal segments in the treatment group with GQD had increased significantly （P〈0.05） compared with the model group. The number of lactobacilli in various intestinal segments was reduced by antibiotics. GQD had regulation effects on the number of lactobacilli in antibiotic associated diarrhea model.

关 键 词：葛根芩连汤 抗生素相关腹泻 乳酸杆菌属 荧光定量PCR 

分 类 号：S811.5[农业科学—畜牧学]