柴贝止痫汤调节海人酸致痫大鼠脑组织耐药蛋白MRP2表达的研究  

Study on regulation of Chaibei Zhixian Decoction on expression of MRP2 protein in brain tissue of kainic acid-induced epilepsy rats

作　　者：王潇慧[1] 鄢泽然[2] 张青[3] 聂莉媛 孙金[1] 张媛[1] 刘金民[5] 

机构地区：[1]北京中医药大学,北京100029 [2]中日友好医院,北京100029 [3]浙江省中医院,杭州321017 [4]北京市昌平区中西医结合医院,北京102208 [5]北京中医药大学第三附属医院,北京100029

出　　处：《中华中医药杂志》2016年第12期4961-4965,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：2012年教育部高等学校博士学科点专项科研基金项目(No.20120013110011);国家自然科学基金项目(No.81373586)~~

摘　　要：目的:观察中药柴贝止痫汤对海人酸(KA)致痫大鼠模型脑组织耐药蛋白MRP2(MRP2)的作用。方法:采用侧脑室注射KA的方法建立癫痫大鼠模型,分为正常组、假手术组、模型组、卡马西平组、中药组和中西药组共6组,分别于给药1个月及3个月后取新鲜脑组织进行蛋白质印迹法(Western Blot)以及实时荧光定量PCR(RT-PCR)来观察各组大鼠脑内MRP2表达的情况并比较组间差异。结果:与模型组比较,1月及3月正常组、假手术组大鼠海马组织中MRP2的表达显著降低(P<0.05),中药组及中西药组表达显著降低(P<0.05)。结论:长期服用卡马西平使MRP2在KA致痫大鼠脑组织中表达升高,而中药柴贝止痫汤可降低KA致痫大鼠脑组织中MRP2的表达。Objective： To observe the effect of Chaibei Zhixian Decoction on expression of MRP2 protein in brain tissue of kainic acid（KA）-induced epilepsy rats. Methods： Epilepsy rat models were established by injecting KA in the lateral ventricle. Rats were divided into normal group, sham operation group, model group, carbamazepine group, traditional Chinese medicine group and western medicine group. Fresh brain tissue was taken in 1 and 3 months after administration. The expression of MRP2 was detected by Western Blot and RT-PCR. Results： Compared with the model group, the expression of MRP2 in the hippocampus of epilepsy rats in the normal group and the sham operation group decreased in the 1st and 3rd months（P〈0.05）, the difference in expression of carbamazepine group was not significant（P〈0.05）, and the expression of traditional Chinese medicine group and western medicine group decreased（P〈0.05）. Conclusion： Long-term administration of carbamazepine increased the expression of MRP2 in brain tissue of rats with seizures induced by kainic acid, and Chaibei Zhixian Decoction could reduce the expression of MRP2 in brain tissue of rats with seizure induced by KA.

关 键 词：柴贝止痫汤 海人酸 多药耐药相关蛋白 难治性癫痫 

分 类 号：R285.5[医药卫生—中药学]