针刺对强迫游泳应激抑郁模型大鼠海马/血清热休克蛋白70表达的影响  

Effects of acupuncture on the expression of heat shock protein 70 in the hippocampus and serum of rat model of depression induced by force swimming stress

作　　者：郭郁[1] 包伍叶[2] 图娅[1] 邬继红[1] 许明敏[1] 于淼[1] 王瑜[1] 张旭辉[1] 张春涛[1] 赵冰骢 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]内蒙古民族大学附属医院,通辽028007

出　　处：《中华中医药杂志》2016年第12期5015-5018,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81173334;No.81373729)~~

摘　　要：目的:从中枢/外周观察针刺对急性强迫应激游泳模型大鼠海马/血清热休克蛋白70(HSP70)蛋白表达影响,探究针刺在急性应激下的抗损伤作用及是否涉及c-Jun氨基末端激酶(JNK)信号通路,揭示针刺相关急性抗抑郁机制。方法:将36只SD大鼠随机分成空白组、空白+JNK通路阻断剂(SP600125,以下简称SP)组、模型组、模型+SP组、针刺组、针刺+SP组,每组6只,采用强迫游泳应激结合孤养方法造模,针刺干预选取"百会""印堂"穴,每次20min,每天1次,治疗2d。采用酶联免疫吸附(ELISA)方法检测大鼠海马和血清的应激保护因子HSP70的蛋白表达水平。结果:与空白组比较,空白+SP组海马/血清热休克蛋白70表达水平无显著差异,模型组显著升高(P<0.01)。与模型组比较,模型+SP组、针刺组、针刺+SP组海马/血清热休克蛋白70表达水平均显著降低(P<0.01)。结论:针刺干预作用可作为保护因素减轻了急性应激对机体产生的不利影响,可能与抑制大鼠海马JNK信号通路活性有关。Objective： To explore the anti-injury effect of acupuncture in acute stress and whether c-Jun N-terminal Kinase（JNK） was involved based on effects of acupuncture on the expression of heat shock protein 70 in the hippocampus and serum of rat model of depression induced by force swimming stress in central and peripheral, to further investigate the molecular mechanism of acupuncture in the treatment of acute depression. Methods： Thirty-six Sprague-Dawley rats were randomly divided into 6 groups： control group, control＋JNK inhibitor group（SP600125, SP for shot below）, model group, model＋SP group, acupuncture group and acupuncture＋SP group, six rats each group. The rat model of depression was induced by the forced swimming combined with solitary raising method. Acupuncture groups were given acupuncture on acupoints of Baihui（GV 20） and Yintang（GV 29） for 20 min, once a day for 2 days. The contents of HSP70 in rat hippocampus and serum were detected by ELISA. Results： Compared with the control group, no obvious difference in the content of HSP70 in hippocampus and serum was found in control＋SP group, but the contents of HSP70 were increased significantly in the model group（P〈0.01）. Compared with the model group, the contents of HSP70 in hippocampus and serum in model＋SP group, acupuncture group and acupuncture＋SP group were significantly decreased（P〈0.01）. Conclusion： Acupuncture intervention may protect the body from acute stress, the mechanism of which may be related to inhibit the activity of the JNK signal pathway in the hippocampus of rat model of depression induced by forced swimming stress.

关 键 词：针刺 强迫游泳应激 海马 血清 抑郁症 热休克蛋白70 JNK信号通路阻断剂SP600125 

分 类 号：R245[医药卫生—针灸推拿学] R-332[医药卫生—中医临床基础]