化瘀通络中药对糖尿病肾病大鼠瘀血阻络证相关实验室指标的影响  

Effects of herbs for removing blood stasis and obstruction in collaterals on diabetic nephropathy rats with syndrome of static blood blocking collaterals

作　　者：郭倩[1] 陈志强[2] 方敬[1] 陈春宇[1] 刘利飞 王春宁[1] 谢田[1] 孙汝[1] 张肖[1] 徐晶[3] 

机构地区：[1]河北医科大学,石家庄050017 [2]河北省中医院,石家庄050011 [3]河北中医学院,石家庄050200

出　　处：《中华中医药杂志》2016年第12期5188-5191,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81173419;No.81373804)~~

摘　　要：目的:研究化瘀通络中药干预对糖尿病肾病(DN)大鼠瘀血阻络证相关实验室指标的影响,探讨该中药降低DN尿蛋白、减轻肾脏病理损伤可能的作用机制。方法:以高糖高脂饲料联合链脲佐菌素(STZ)35mg/kg造模,成模大鼠予化瘀通络中药灌胃16周。第16周末留取大鼠血、尿、肾组织行进一步检测。结果:与正常组比较,模型组大鼠糖化血红蛋白、血糖、血脂、24h尿蛋白定量水平明显升高(P<0.01),并出现肾小球肥大,基底膜增厚,系膜基质增生等肾脏病理改变;血小板参数中平均血小板体积、血小板分布宽度,血凝中血浆凝血酶原时间、活化部分凝血活酶时间、抗凝血酶Ⅲ及v WF、CD62p表达均有显著差异(P<0.01)。与模型组比较,中药组大鼠上述瘀血阻络证指标均有统计学意义(P<0.05),24h尿蛋白定量水平明显降低(P<0.05),肾脏病理改变减轻。结论:化瘀通络中药具有确切的降低DN大鼠尿蛋白和减轻肾脏病理损伤的作用,这可能与其纠正脂代谢紊乱、改善血液高凝状态、保护内皮完整性以及抑制异常血小板活化有关。Objective： To investigate the effect of herbs for removing blood stasis and obstruction in collaterals on related experimental indicators of diabetic nephropathy（DN） rats with syndrome of static blood blocking collaterals, in order to explore the possible mechanism of the Chinese medicine to reduce the DN urinary protein and reduce renal pathological damage. Methods： The rat models were established by feeding with high-sugar high-fat feed combined with STZ（35mg/kg）, After the success of modeling, rats were treated with herbs for removing blood stasis and obstruction in collaterals for 16 weeks. At the end of the 16 th week, the blood, urine and renal tissues were collected for further examination. Results： Compared with the normal group, the levels of glycosylated hemoglobin, blood sugar, blood lipids and 24 h urinary protein in the model group were significantly higher（P〈0.01）, showed glomerular hypertrophy, basement membrane thickening, mesangial proliferation and other pathological changes, and there were significant differences in mean platelet volume, platelet distribution width, plasma prothrombin time, activated partial thromboplastin time, antithrombin Ⅲ and v WF, CD62 p expression in platelet parameters（P〈0.01）. Compared with the model group, the blood stasis syndrome index of the TCM group were statistically significant（P〈0.05）, 24 h urinary protein levels were significantly lower（P〈0.05）, and renal pathological changes reduced. Conclusion： Herbs for removing blood stasis and obstruction in collaterals has the exact function of reducing urinary protein of DN rats and alleviating renal pathological damage, which may be related to correcting lipid metabolic disorder, improving hypercoagulability state, protecting endothelial integrity and inhibiting abnormal platelet activation.

关 键 词：糖尿病肾病 瘀血阻络证 血小板 血凝 血管性血友病因子 CD62P 

分 类 号：R285.5[医药卫生—中药学]