加味过敏煎拆方组分对膜性肾病大鼠白细胞介素-4、白细胞介素-10等细胞因子的影响  

Effects of different medicinals in Jiawei Guomin Decoction on rats with C-BSA induced nephropathy by analyzing serum cytokines of IL-4, IL-10, etc.

作　　者：尼日特 杨巧芳[1] 薛昕[1] 田佳佳[1] 

机构地区：[1]内蒙古医科大学,呼和浩特010110

出　　处：《中华中医药杂志》2016年第12期5277-5280,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金青年基金项目(No.81102583)~~

摘　　要：目的:按照调理开合、健脾益肾、活血化瘀法把加味过敏煎拆分成三大组分,观察分析其不同组分对膜性肾病大鼠蛋白尿及其相关细胞因子的影响。方法:用阳离子化牛血清蛋白尾静脉注射造成大鼠膜性肾病模型,用加味过敏煎不同组分进行干预,用考马斯亮兰法检测24小时尿蛋白定量,用ELISA法检测血清中IL-4、IL-10、IFN-γ、TGF-β的含量。结果:加味过敏煎各组分均对蛋白尿有不同程度的缓解作用,其中以调理开合组分和活血化瘀组分较为明显;活血化瘀组分对IL-4、IL-10、IFN-γ作用较明显,对TGF-β无明显作用;健脾益肾组分对IFN-γ作用较明显。结论:加味过敏煎各组分可改善膜性肾病大鼠的蛋白尿,可调节相关细胞因子的水平,不同药物组分对不同细胞因子显示出了各自的优势作用,说明各组分发挥作用的路径有所不同。Objective： To observe and research the effect of Jiawei Guomin Decoction on proteinuria and its related cytokines of membranous nephropathy in rats, which were divided into threeprescription components according to their functions of regulating opening and closing of sweat pores, invigorating spleen and kidney, promoting blood circulation to remove stasis. Methods： The model rats with membranous nephropathy induced by tail vein injecting cationic bovine serum protein（C-BSA）, were treated by the different prescription components of Jiawei Guomin Decoction. The 24 hour urinary proteins were respectivelydetected by Coomassie brilliant blue method, the IL-4, IL-10, IFN-γ, TGF-β in serum by ELISA.Results： The prescription components of Jiawei Guomin Decoction have significant relieving effect on the 24 hour urinary proteins, but it shows more effective for components of regulating opening and closing of sweat pores, and promoting blood circulation to remove stasis. The component of promoting blood circulation to remove stasis has significant effect on the IL-4, IL-10, IFN-‘, but no obvious effect on TGF-β in serum; the component of invigorating spleen and kidney has significant effect on IFN-γ. Conclusion： The prescription components of Jiawei Guomin Decoction can reduce proteinuria of membranous nephropathy in rats and regulate the related cytokines, and different prescription components show advantages respectively on different cytokines, which show different components play a role in different paths.

关 键 词：加味过敏煎 组分 膜性肾病 白细胞介素 

分 类 号：R285.5[医药卫生—中药学]